| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,380,362 | 0 | C | T | 53.6% | ‑0.8 / 31.2 | 28 | intergenic (+52/‑51) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
| Reads supporting (aligned to +/- strand): ref base C (6/7); new base T (15/0); total (21/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.45e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.01e-06 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAA > NZ_CP009273/2380275‑2380451 | gATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATGCACATTTTATTTTccc < 1:120045/90‑1 (MQ=255)gATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATGCACATTTTATTTTccc < 2:1047358/90‑1 (MQ=255) tGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCCGAAAAAAAGACAAGCACAATTTTTTTTTCCTc > 1:886643/1‑90 (MQ=255) ggTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCCGAAAAAACGACAAGCACATTTTTTTTTtccccc > 1:856204/1‑87 (MQ=255) ggTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCCGAAAAAAAGACAAGCACATTTTTTTTTTCCTCa > 1:217111/1‑90 (MQ=255) ggTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCAGAAAAAGCGACATGGACATTTTTTTTTTCCTcc > 1:369024/1‑89 (MQ=255) ggTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCAAAAAAAAAGATAAACACAATTTTTTTTTCCTcc > 1:1267879/1‑89 (MQ=255) gtgtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCAAAAAAAAAGACAAGCAAATTTTTTTTTTTCTCAt > 1:692769/1‑90 (MQ=255) gtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCAGAAAAAAAGGCATGCACATTTTTTTTTTCCTCctct > 1:516704/1‑90 (MQ=255) ttATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCAGAAAAAAAGACATGCACATTTTTTTTTTTCTCctctt > 1:119690/1‑89 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCTGAAAAAAAGACAAGCAAAATTTTTTTTTTCTCCTCTGttttttt > 2:28964/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCCGAAAAAAAGACAAGCACAATTTTTTTTTTCTCCTCtttttttaa > 2:442801/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCAGAAAAAAAGACAAGCACATTTTTTTTTTTCTCATTTGTTTTTAg > 1:966558/1‑90 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCAGAAAAAAAGAAAAGAACATTTTTTTTTTTCTCCTCTGtttttat > 2:881828/1‑87 (MQ=255) tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑GCCAGAAAAAAAGAAAAACAAATTTTTTTTTTTCTCCTCtttttttat > 2:893369/1‑87 (MQ=255) acaacaGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGaaa < 2:119690/90‑1 (MQ=255) aGTAAATAATAAAAATTTCTCGAGGCGGGCTCGCGAAAAA‑CGAAAAGAACATTTTATTTTTCCCCCTCTGTTTTTAGATGCGAAACACGa > 2:356206/1‑90 (MQ=255) aGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGa < 1:601527/90‑1 (MQ=255) aaaTAATAAAAATTTCTCGAGGCGGG‑GCCAGAAAAAAAAACAAGCACAATTTTTTTTTCCTCCTCTGTTTTTAGAAGGGAAAAAAGaaaa > 2:297752/1‑89 (MQ=255) ataaAAATTTCTCGAGGCGGG‑TCTAGAAAAAACGACATGCACATTTTATTTTTCCTCATCTGTTTTTAGATGCGAAAAATGAAATTTaaa > 1:509939/1‑89 (MQ=255) aTTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACggagga < 2:1267879/90‑1 (MQ=255) aTTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACggagga < 2:1117443/90‑1 (MQ=255) ggCGGG‑TCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAaaataaat < 2:49897/90‑1 (MQ=255) aaGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCt > 1:581382/1‑90 (MQ=255) aGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTa > 1:208518/1‑90 (MQ=255) cATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTg > 1:262067/1‑90 (MQ=255) cATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTg > 2:751846/1‑90 (MQ=255) ttttattttCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAAtt < 1:442801/90‑1 (MQ=255) attttCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACt < 1:893369/90‑1 (MQ=255) tCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGa > 2:1073466/1‑90 (MQ=255) tCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGa > 2:375757/1‑90 (MQ=255) cccTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGaa < 1:438653/90‑1 (MQ=255) cccTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGaa < 2:288160/90‑1 (MQ=255) cccTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGaa < 1:986019/90‑1 (MQ=255) | GATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGG‑TCCAGAAAAAGCGACATGCACATTTTATTTTCCCTCATCTGTTTTAAGATGCGAAACATGAAATTCAACGGAGGAAAAATAAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAA > NZ_CP009273/2380275‑2380451 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |