Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,726,562	(T)5→4	coding (971/2574 nt)	clpB ←	ATP‑dependent chaperone ClpB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,726,558	0	T	.	100.0%	92.7 / NA	22	coding (975/2574 nt)	clpB	ATP‑dependent chaperone ClpB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (12/10);  total (12/10)

ACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGT  >  NZ_CP009273/2726480‑2726643                                                                               |                                                                                      aCGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATC‑TTTTCAATGTAc                                                                           >  2:99877/1‑90 (MQ=255) aCGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATC‑TTTTCAATGTAc                                                                           >  2:116776/1‑90 (MQ=255)           gCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATC‑TTTTCAATGTACTGGCGATAtt                                                                 >  1:193984/1‑90 (MQ=255)                    tCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATC‑TTTTCAATGTACTGGCGATATTCGTCAAGcg                                                        <  1:107567/90‑1 (MQ=255)                        cAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATC‑TTTTCAATGTACTGGCGATATTCGTCAAGcgtcgt                                                    >  2:191816/1‑90 (MQ=255)                         aaCAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATC‑TTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTg                                                   <  2:354940/90‑1 (MQ=255)                         aaCAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATC‑TTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTg                                                   <  2:344097/90‑1 (MQ=255)                         aaCAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATC‑TTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTg                                                   <  2:193984/90‑1 (MQ=255)                              aaGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATC‑TTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCAcc                                              <  1:306310/90‑1 (MQ=255)                               aGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATC‑TTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCt                                             >  1:311928/1‑90 (MQ=255)                               aGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATC‑TTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCt                                             >  2:269924/1‑90 (MQ=255)                                   tCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATC‑TTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGc                                         <  1:432508/90‑1 (MQ=255)                                   tCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATC‑TTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGc                                         <  2:26103/90‑1 (MQ=255)                                             acacTTTCTGGAAACGACGTTCCAGCGCAGCATC‑TTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTc                               <  2:311928/90‑1 (MQ=255)                                              cacTTTCTGGAAACGACGTTCCAGCGCAGCATC‑TTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTca                              >  1:349960/1‑90 (MQ=255)                                                   tcgggAAACGACGTTCCAGCGCAGCATC‑TTTTCAATGAACTGGCGATATTCGTCAAGCGCCGGGGCACCAACGATGTTCAATTaaccact                         >  2:424374/4‑89 (MQ=255)                                                            gacgTTCCAGCGCAGCATC‑TTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTg                                                   <  1:461328/55‑1 (MQ=255)                                                            gacgTTCCAGCGCAGCATC‑TTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTg                                                   >  2:461328/1‑55 (MQ=255)                                                                       gcagcaTC‑TTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGc      >  1:109326/1‑89 (MQ=255)                                                                       gcagcaTC‑TTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGc      <  2:109326/89‑1 (MQ=255)                                                                       gcagcaTC‑TTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCa     >  1:32412/1‑90 (MQ=255)                                                                          gcaTC‑TTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGt  >  2:139129/1‑90 (MQ=255)                                                                               |                                                                                      ACGCAGAATCGCAATGGTATCTTCAACAGAAGGCTCGGCAACAAACACTTTCTGGAAACGACGTTCCAGCGCAGCATCTTTTTCAATGTACTGGCGATATTCGTCAAGCGTCGTGGCACCTACGCAGTGCAATTCACCACGCGCCAGCGCCGGTTTCAGCATGT  >  NZ_CP009273/2726480‑2726643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑