Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,122,270	(A)6→5	coding (9/924 nt)	yceM →	Gfo/Idh/MocA family oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,122,265	0	A	.	100.0%	20.2 / NA	6	coding (4/924 nt)	yceM	Gfo/Idh/MocA family oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (2/4);  total (2/4)

AGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGA  >  NZ_CP009273/1122178‑1122349                                                                                        |                                                                                     aGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGaaa                                                                                    <  2:313730/90‑1 (MQ=255)  ggCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGaaaa                                                                                   >  1:281336/1‑90 (MQ=255)                  cTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTa                                                                  >  1:287145/1‑90 (MQ=255)                     gAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTg                                                               <  2:259253/90‑1 (MQ=255)                         tCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGAt                                                           >  1:316015/1‑90 (MQ=255)                         tCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGAt                                                           <  1:426388/90‑1 (MQ=255)                         tCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTG‑AAAAATTACGTATCGGCGTAGTGGGAt                                                           <  2:345076/90‑1 (MQ=255)                                                                                  aaaGTG‑AAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGa  <  2:58388/90‑1 (MQ=255)                                                                                        |                                                                                     AGGCCCGCGTCGAAGCCCTGGAAATCGAAGTGGCAGAACTGAAACAGCGTCTTGATTCGTTGCTGGCCCATCTGGGAGATTAAAGTGAAAAAATTACGTATCGGCGTAGTGGGATTAGGTGGCATTGCGCAAAAAGCGTGGTTACCGGTGCTGGCGGCAGCGTCTGACTGGA  >  NZ_CP009273/1122178‑1122349

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑