Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,223,232	(A)8→7	coding (63/294 nt)	BW25113_RS06140 →	YcgL domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,223,225	0	A	.	100.0%	33.5 / NA	9	coding (56/294 nt)	BW25113_RS06140	YcgL domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (6/3);  total (6/3)

GAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGAT  >  NZ_CP009273/1223160‑1223310                                                                  |                                                                                      gagTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCG‑AAAAAAAGACGATTTTTCGCGTGtt                                                              <  1:215775/90‑1 (MQ=255)   gTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCG‑AAAAAAAGACGATTTTTCGCGTGTTcc                                                            >  1:178660/1‑90 (MQ=255)                       aTTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCG‑AAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGtt                                        >  1:236231/1‑90 (MQ=255)                       aTTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCG‑AAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGtt                                        <  2:267544/90‑1 (MQ=255)                                            gACCAGACCTATTTATATGTCG‑AAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCga                   >  1:90829/1‑90 (MQ=255)                                                 gACCTATTTATATGTCG‑AAAAAAAGACGATTTTTCGCgt                                                                 >  1:86853/1‑39 (MQ=39)                                                 gACCTATTTATATGTCG‑AAAAAAAGACGATTTTTCGCgt                                                                 <  2:86853/39‑1 (MQ=39)                                                     tATTTATATGTCG‑AAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGc          >  2:196646/1‑90 (MQ=255)                                                             tGTCG‑AAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGAt  >  1:477245/1‑90 (MQ=255)                                                                  |                                                                                      GAGTAAAAGTATGTTTTGTGTGATTTATCGAAGCAGCAAGCGTGACCAGACCTATTTATATGTCGAAAAAAAAGACGATTTTTCGCGTGTTCCTGAGGAACTGATGAAAGGTTTTGGTCAGCCTCAGTTAGCGATGATTCTGCCGCTGGAT  >  NZ_CP009273/1223160‑1223310

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑