| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,276,251 | Δ1 bp | coding (932/3744 nt) | narG → | nitrate reductase subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,276,249 | 0 | G | . | 94.4% | 68.1 / ‑2.1 | 18 | coding (930/3744 nt) | narG | nitrate reductase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base . (11/6); total (11/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.89e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGC > NZ_CP009273/1276162‑1276318 | cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggc > 2:263523/1‑89 (MQ=255)cacaCCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATggc < 1:93417/90‑2 (MQ=255) aCGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAAtgct > 2:85062/1‑90 (MQ=255) aCGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAAtgct > 2:449123/1‑90 (MQ=255) aCGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAAtgct > 2:200249/1‑90 (MQ=255) aCGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAAtgct > 2:124656/1‑90 (MQ=255) aCGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAAtgct > 1:95002/1‑90 (MQ=255) cGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAAtgctg > 1:156733/1‑90 (MQ=255) cGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAAtgctg > 1:71863/1‑90 (MQ=255) cTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCg > 1:401686/1‑90 (MQ=255) aaaTCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGa < 2:140707/90‑1 (MQ=255) aaCTGTGCGATCTGTGGCTGGCACCGAACCAGGGCCCCGATGCGGCAATGGCGCTGGCGATGGGCCACGCAATGGTGCGTGAAATCcccc < 2:409419/90‑1 (MQ=38) gtgCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCACCTCGAc < 2:409963/90‑1 (MQ=255) gtgCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCACCTCGAc > 2:415547/1‑90 (MQ=255) tgCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCACCTCGACa > 1:426593/1‑90 (MQ=255) aaCAGGGCACCGATGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGa < 1:415547/90‑1 (MQ=255) tGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGc < 2:156733/90‑1 (MQ=255) tGCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGc < 1:124656/90‑1 (MQ=255) gCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCAcc > 2:264021/1‑48 (MQ=39) gCGGCAATGGCGCTGGCGAT‑GGCCACGTAATGCTGCGTGAATTCCAcc < 1:264021/48‑1 (MQ=39) | CACACCAGACTACGCTGAAATCGCCAAACTGTGCGATCTGTGGCTGGCACCGAAACAGGGCACCGATGCGGCAATGGCGCTGGCGATGGGCCACGTAATGCTGCGTGAATTCCACCTCGACAACCCAAGCCAGTATTTCACCGACTATGTGCGTCGC > NZ_CP009273/1276162‑1276318 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |