Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,759,232	(G)6→7	intergenic (‑97/‑45)	yfjM ← / → rnlA	DUF1524 domain‑containing protein/type II toxin‑antitoxin system toxin mRNA endoribonuclease RnlA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,759,226	1	.	G	100.0%	22.3 / NA	8	intergenic (‑91/‑51)	yfjM/rnlA	DUF1524 domain‑containing protein/type II toxin‑antitoxin system toxin mRNA endoribonuclease RnlA
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (7/1);  total (7/1)

GACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAA  >  NZ_CP009273/2759142‑2759297                                                                                      |                                                                        gACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑ggggg                                                                    <  2:386551/90‑1 (MQ=255)  aCTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑gggggg                                                                   <  1:433904/90‑1 (MQ=255)      cccccccGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑gggggggtat                                                               <  1:217644/90‑5 (MQ=255)      cccccccGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑gggggggtat                                                               <  1:40382/90‑5 (MQ=255)               tAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGGTATTATTGTaga                                                       >  2:470470/1‑90 (MQ=255)                               ataGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGGTATTATTGTAGAGTTTCCCCATATGttt                                       >  2:217361/1‑90 (MQ=255)                                    tGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATg                                  >  2:281654/1‑90 (MQ=255)                                         tGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATc                             >  2:48091/1‑90 (MQ=255)                                          gCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATcc                            >  2:168179/1‑90 (MQ=255)                                          gCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATcc                            >  2:53984/1‑90 (MQ=255)                                                      ggTGGCTAAATTTAGCAAATCAATACACTTCAGGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACaa                >  1:294468/1‑90 (MQ=255)                                                                    gCAAATCAATACACTTCAGGGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACaaa  <  1:470470/90‑1 (MQ=255)                                                                                      |                                                                        GACTGCCCCCCCGGTAGACGATCCTGCCCTATAGTTGGAGTGCCCCCTATTCAGGTGGCTAAATTTAGCAAATCAATACACTTCA‑GGGGGGTATTATTGTAGAGTTTCCCCATATGTTTCTATGGGATCCAGGAAATGACAATCAGGAGTTACAAA  >  NZ_CP009273/2759142‑2759297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑