| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,738,402 | 0 | A | C | 55.0% | 1.1 / 11.5 | 21 | D80A (GAC→GCC) | yiaN | 2,3‑diketo‑L‑gulonate transporter large permease YiaN |
| Reads supporting (aligned to +/- strand): ref base A (6/3); new base C (0/11); total (7/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.17e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.33e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTT > NZ_CP009273/3738322‑3738484 | cTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGGGAAATCATGAATGCGGGGGGCCTGTCAAAACGCATTGTTGACCTGCCGAt > 2:184764/1‑90 (MQ=255) tgctgGCGATTCCGTTCTTTGTGCTGGCGGGGGAAATCATGAAAGCGGGCGGCCTGTCAAAACGCATTGTTGGCCTGCCCCTGAAACtgg > 2:368388/1‑90 (MQ=255) gctgGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTTACCTGCCGATGAAACtggg > 1:13735/1‑89 (MQ=255) ctgGCGATCCCGTTCTTTGTGCGGGGGGGTGAAATCATGAATGCGGGCGGCTTGTAAAAACGCATTGTTGACCTGCCGATGAAACtggtg < 1:415123/90‑1 (MQ=255) cgggCGATTCCGTTCTTTGTGGTGGGGGGTGAAATCATGAATGGGGGGGGCTTGTAAAAACGCATTGTTGCCCTGCCGATGAAACtggtg < 2:415984/88‑1 (MQ=255) gATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGGAACTGggggggga > 1:254398/1‑88 (MQ=255) tttCCGTTTTTTGTGCTGGGGGGTAAAATCATGAAGGGGGGGGGTTTGTAAAACCGCATTGTTGCCCTGCCGATGAAACTGGTGGGGCAt < 2:287093/89‑1 (MQ=255) tttGTGCTGGGGGGTAAAATCATGAATGCGGGCGGCTTGTAAAACCGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGc < 1:226029/90‑1 (MQ=255) tttGGGCTGGCGGGTAAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGCCCTGCCGATGAAACTGGTGGGGCATAAACCAGGc < 2:166913/90‑1 (MQ=255) gtgCGGGGGGGTAAGATCATCAAGGCGCGGGGCTTCTCAAACCGCCTTTTGGCCCTGCCGATGAAACTGGTGGGGCATAAACCAGGCggg < 2:164534/90‑1 (MQ=255) tggtGGGGGGTGAAATAATGAATGGGGGGGGTTTGTCAAAACGCTTTTTTGCCCTGCCGATGAAACTGGTGGGGCATAAACCAGGCgggc < 1:357699/87‑1 (MQ=255) gggggTAAAATCATGAAGGGGGGGGGTTTGTAAAACCGCTTTGTTGCCCTGCCGATGAAACTGGTGGGGCATAAACCAGGCgggctgggc < 2:216639/88‑1 (MQ=255) gCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAAAGCATTGTTGACCTGCCGATGAAAATGGGGGGGCATAAAACAGGCgggctgggc > 1:216639/1‑90 (MQ=255) aaggcgggcggCTAGTCAAAAAGCCTTGTTGCCCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTg < 1:228870/87‑1 (MQ=255) tGCGGGGGGCTTGTCAAACCGCTTTGTGGCCCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGc < 2:431503/90‑1 (MQ=255) cggcTTGTCAAAACGCATTGTTGCCCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTAt < 1:59335/90‑1 (MQ=255) ccGCTTTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAg < 2:254398/89‑1 (MQ=255) gCATTGTTGCCCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGcc < 1:287040/90‑1 (MQ=255) cATTGTTGCCCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCg < 2:415716/62‑1 (MQ=255) cATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCg > 1:415716/1‑62 (MQ=255) aTTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCtt > 1:454363/1‑90 (MQ=255) | CTTCTCCCTGCTGGCGATTCCGTTCTTTGTGCTGGCGGGTGAAATCATGAATGCGGGCGGCTTGTCAAAACGCATTGTTGACCTGCCGATGAAACTGGTGGGGCATAAACCAGGCGGGCTGGGCTACGTCGGCGTGCTGGCAGCTATGATTATGGCGAGCCTT > NZ_CP009273/3738322‑3738484 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |