Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,761,086 A→C R619R (CGT→CGG)  ydiJ ← FAD‑binding and (Fe‑S)‑binding domain‑containing protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,761,0860AC90.0% 26.2 / ‑4.7 10R619R (CGT→CGG) ydiJFAD‑binding and (Fe‑S)‑binding domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (3/6);  total (3/7)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACA  >  NZ_CP009273/1761010‑1761163
                                                                            |                                                                             
aCGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCAc                                                                  <  1:309800/90‑1 (MQ=255)
  gTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGc                                                                >  1:471467/1‑90 (MQ=255)
      gCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCa                                                            >  1:439302/1‑90 (MQ=255)
               cGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTgcgc                                                   <  1:428150/90‑1 (MQ=255)
                 ccGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTgcgcgc                                                 <  1:463561/90‑1 (MQ=255)
                 ccGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTgcgcgc                                                 <  2:146213/90‑1 (MQ=255)
                                        cAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGtt                          >  2:167285/1‑90 (MQ=255)
                                                          gccgcGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGa        <  1:167890/90‑1 (MQ=255)
                                                          gccgcGATCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGa        <  2:56975/90‑1 (MQ=255)
                                                                aTCCGCCAACAACCGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCgacagaca  <  1:466918/90‑1 (MQ=255)
                                                                            |                                                                             
ACGTCCGCAAACTGACGCCGGATTCAGGCAGTTCTTGTTCCAGTTTGAGTGGATCAACGCCGCGATCCGCCAACAAACGCAGCCATTCACGCACCAGCGTTGCGCGCCCTTTCGGTGAATGAATCCGGTTCTGGGTGATCTTCATCGACGGACA  >  NZ_CP009273/1761010‑1761163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑