Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,782,276	T→G	V192G (GTG→GGG)	ppsR →	posphoenolpyruvate synthetase regulatory kinase/phosphorylase PpsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,782,276	0	T	G	87.5%	18.3 / ‑3.8	8	V192G (GTG→GGG)	ppsR	posphoenolpyruvate synthetase regulatory kinase/phosphorylase PpsR
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base G (5/2);  total (6/2)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGCAATGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGTGCTACCCGCGTCGCTCAAACCGCTTCAGCATAAATTGTTCGGCCTGACTATCGACCCGGAACGTCTGGCGGCGATTCGCGAGGAACGT  >  NZ_CP009273/1782210‑1782364                                                                   |                                                                                         tGGCAATGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGc                                                                   >  2:282008/1‑90 (MQ=255) tGGCAATGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGc                                                                   >  2:413860/1‑90 (MQ=255)   gCAATGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCtt                                                                 <  1:413860/90‑1 (MQ=255)      aTGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAg                                                              >  1:266277/1‑90 (MQ=255)      aTGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAg                                                              >  2:68965/1‑90 (MQ=255)             ttGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAGCATAAAt                                                       >  1:295489/1‑90 (MQ=255)                      gcgcgGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGGGCTACCCGCGTCGCTCAAACCGCTTCAGCATAAATTGTTCGGcc                                              <  1:15367/90‑1 (MQ=255)                                                                  gTGCTACCCTCGTCGCTCAAACCGCTTCAGCATAAATTGTTCCGCCTGACTAGCGACCCGGAACGTCTGGGGGCGAGTCGCGAAGAACGt  >  2:244885/1‑90 (MQ=255)                                                                   |                                                                                         TGGCAATGCAGTTTGGTATCCGCGCGGCAAACTACCCCTTTATTGCCGACGATATGGATAATCTGGTGCTACCCGCGTCGCTCAAACCGCTTCAGCATAAATTGTTCGGCCTGACTATCGACCCGGAACGTCTGGCGGCGATTCGCGAGGAACGT  >  NZ_CP009273/1782210‑1782364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑