Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,796,543	G→C	G95G (GGC→GGG)	thrS ←	threonine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,796,543	0	G	C	100.0%	62.3 / NA	21	G95G (GGC→GGG)	thrS	threonine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (6/15);  total (6/15)

ATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATGA  >  NZ_CP009273/1796457‑1796619                                                                                       |                                                                             aTCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGCCCg                                                                           >  2:226121/1‑90 (MQ=255)      cTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGCCCGATTGc                                                                      <  1:32941/90‑1 (MQ=255)        tctcGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGCCCGATTGCCa                                                                    >  2:349910/1‑90 (MQ=255)                          cTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGCCCGATTGCCATTTTGGTATGCGGCCaaa                                                  <  2:282496/90‑1 (MQ=255)                                  gTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGCCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTa                                          >  1:420195/1‑90 (MQ=255)                                       cGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGCCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATcgc                                     <  1:178822/90‑1 (MQ=255)                                         tGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGCCCGATTGCCATTTTGGTATGCCGCCAAAGTTGTTTAATCGCGt                                   <  2:325650/90‑1 (MQ=255)                                          gCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGCCCGATTGCCATTTTGGTATGCGGCCAAAgt                                                >  1:380517/1‑76 (MQ=255)                                          gCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGCCCGATTGCCATTTTGGTATGCGGCCAAAgt                                                <  2:380517/76‑1 (MQ=255)                                          gCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGCCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTg                                  <  2:126843/90‑1 (MQ=255)                                            ggTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGCCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGcc                                <  1:326341/90‑1 (MQ=255)                                                      aaCGTCGTAATAAAAACCGTTGTCAATAACCGGCCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTg                      <  1:420941/90‑1 (MQ=255)                                                         gtcgtAATAAAAACCGTTGTCAATAACCGGCCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTgcgc                   <  1:190447/90‑1 (MQ=255)                                                         gtcgtAATAAAAACCGTTGTCAATAACCGGCCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTgcgc                   <  2:63581/90‑1 (MQ=255)                                                         gtcgtAATAAAAACCGTTGTCAATAACCGGCCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTgcgc                   <  2:356110/90‑1 (MQ=255)                                                           agtAATAAAAACCGTTGTCAATAACCGGCCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGcac                 <  2:314367/89‑1 (MQ=255)                                                            gtAATAAAAACCGTTGTCAATAACCGGCCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGcaca                >  1:95673/1‑90 (MQ=255)                                                                        gTTGTCAATAACCGGCCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTg                                  >  1:475359/1‑60 (MQ=255)                                                                        gTTGTCAATAACCGGCCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTg                                  <  2:475359/60‑1 (MQ=255)                                                                          tGTCAATAACCGGCCCGCTTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATGa  <  1:315598/90‑1 (MQ=255)                                                                          tGTCAATAACCGGCCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATGa  <  2:95673/90‑1 (MQ=255)                                                                                       |                                                                             ATCCGCTTCTCGAGTGCTTCGACATCTTCCTGGGTTAACGTGCGGTCAAGATCAACGTCGTAATAAAAACCGTTGTCAATAACCGGGCCGATTGCCATTTTGGTATGCGGCCAAAGTTGTTTAATCGCGTGCCCTAACAGGTGCGCACAGGAGTGACGAATGA  >  NZ_CP009273/1796457‑1796619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑