Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,854,126	T→C	M121V (ATG→GTG)	ydjK ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,854,126	0	T	C	100.0%	44.4 / NA	14	M121V (ATG→GTG)	ydjK	MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (3/11);  total (3/11)

CCACGATTTCTACCGGGCATATATTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCATCACAAAACGGCAGGCAATAAGGAAATCCATATTCGGTGAGAAAGCACCGACAACCATCGAGGCAATATGAATGG  >  NZ_CP009273/1854050‑1854200                                                                             |                                                                           ccACGATTTCTACCGGGCATATATTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACggcag                                                               <  2:475758/90‑1 (MQ=255)           tACCGGGCATATATTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGa                                                     <  1:423888/90‑1 (MQ=255)            aCCGGGCATATATTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGaa                                                    <  2:101894/90‑1 (MQ=255)                    tatatTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGa                                                     >  1:218933/1‑81 (MQ=255)                    tatatTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGa                                                     <  2:218933/81‑1 (MQ=255)                    tatatTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCatat                                            >  2:18530/1‑90 (MQ=255)                       atTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATTCg                                         <  2:400626/90‑1 (MQ=255)                                   cAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATTCGGTGAGAAAGCAc                             <  2:217791/90‑1 (MQ=255)                                             cGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGC‑GGCAATAAGGAAATCCATATTCGGTGAGAAAGCACCGACAACCATc                  <  2:58346/90‑1 (MQ=255)                                                     gTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATTCGGTGAGAAAGCACCGACAACCATCGAGGCaa           <  1:247239/90‑1 (MQ=255)                                                      tAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATTCGGTGAGAAAGCACCGACAACCATCGAGGCAat          <  1:256502/90‑1 (MQ=255)                                                            tCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATTCGGTGAGAAAGCACCGACAACCATCGAGGCAATATGAAt    >  2:457020/1‑90 (MQ=255)                                                            tCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATTCGGTGACAAAGCACCGACAACCATCGAGGCAATATGAAt    <  1:384122/90‑1 (MQ=255)                                                              ccAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATTCGGTGAGAAAGCACCGACAACCATCGAGGCAATATGAATgg  <  2:113490/90‑1 (MQ=255)                                                                             |                                                                           CCACGATTTCTACCGGGCATATATTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCATCACAAAACGGCAGGCAATAAGGAAATCCATATTCGGTGAGAAAGCACCGACAACCATCGAGGCAATATGAATGG  >  NZ_CP009273/1854050‑1854200

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑