Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,174,381	T→G	C270G (TGC→GGC)	yegU →	ADP‑ribosylglycohydrolase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,174,381	0	T	G	100.0%	64.6 / NA	20	C270G (TGC→GGC)	yegU	ADP‑ribosylglycohydrolase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/11);  total (9/11)

GGTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGC  >  NZ_CP009273/2174296‑2174454                                                                                      |                                                                          ggTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGgcgc                                                                       <  2:121590/90‑1 (MQ=255) ggTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGgcgc                                                                       <  1:98127/90‑1 (MQ=255)  gTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGcc                                                                      >  2:50314/1‑90 (MQ=255)                   cAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCt                                                     >  1:269697/1‑90 (MQ=255)                        cTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTggcg                                                >  1:43635/1‑90 (MQ=255)                               gTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGacac                                         >  2:442757/1‑90 (MQ=255)                               gTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGacac                                         <  1:468467/90‑1 (MQ=255)                               gTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGACCTGTGCGCTAACCTTGGCGGCGacac                                         >  2:468467/1‑90 (MQ=255)                                   gttccgtGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGac                                     <  1:193661/90‑1 (MQ=255)                                    ttccgtGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGaca                                    <  2:405505/90‑1 (MQ=255)                                    ttccgtGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGaca                                    >  1:405505/1‑90 (MQ=255)                                    ttccgtGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGaca                                    >  2:5598/1‑90 (MQ=255)                                     tccgtGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGacac                                   <  2:94814/90‑1 (MQ=255)                                        gtGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCAt                                >  1:356156/1‑90 (MQ=255)                                         tGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATc                               <  1:442757/90‑1 (MQ=255)                                         tGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATc                               <  2:43635/90‑1 (MQ=255)                                                       ggTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGaca                                    <  2:155903/71‑1 (MQ=255)                                                       ggTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGaca                                    >  1:155903/1‑71 (MQ=255)                                                         ttGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACgg               <  2:269697/90‑1 (MQ=255)                                                                      aaCCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGgcgc  <  1:448603/90‑1 (MQ=255)                                                                                      |                                                                          GGTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGC  >  NZ_CP009273/2174296‑2174454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑