Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,739,096	T→C	E61G (GAA→GGA)	rimM ←	ribosome maturation factor RimM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,739,096	0	T	C	100.0%	29.4 / NA	10	E61G (GAA→GGA)	rimM	ribosome maturation factor RimM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/4);  total (6/4)

GTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGT  >  NZ_CP009273/2739044‑2739175                                                     |                                                                                gTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGAt                                            >  1:185851/1‑90 (MQ=255)                                 gATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGAt                                            <  1:11776/58‑1 (MQ=255)                                 gATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGAt                                            >  2:11776/1‑58 (MQ=255)                                 gATTGTGGTGCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCgg            >  2:6885/1‑90 (MQ=255)                                     gtggtgCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGAcc                                                        <  1:158224/42‑1 (MQ=255)                                     gtggtgCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGAcc                                                        >  2:158224/1‑42 (MQ=255)                                     gtggtgCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCg                                                      >  1:134355/1‑44 (MQ=255)                                     gtggtgCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCg                                                      <  2:134355/44‑1 (MQ=255)                                       ggtgCTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCtt      >  2:328112/1‑90 (MQ=255)                                           cTTCCAGCTTCCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGt  <  2:478876/90‑1 (MQ=255)                                                     |                                                                                GTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGT  >  NZ_CP009273/2739044‑2739175

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑