Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,849,816	T→C	E117G (GAA→GGA)	ygbA ←	nitrous oxide‑stimulated promoter family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,849,816	0	T	C	100.0%	34.3 / NA	12	E117G (GAA→GGA)	ygbA	nitrous oxide‑stimulated promoter family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/6);  total (6/6)

CACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATC  >  NZ_CP009273/2849732‑2849899                                                                                     |                                                                                    cACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATCCATGa                                                                                >  1:211711/1‑90 (MQ=255) cACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATCCATGa                                                                                >  1:229837/1‑90 (MQ=255) cACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATCCATGa                                                                                >  1:374939/1‑90 (MQ=255)              gCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGACCTGCCTGTCCGGCATACTATCCATGAGGTTTTTTCGGAc                                                                   <  1:365803/90‑1 (MQ=255)                                  cATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCt                                               <  1:9356/90‑1 (MQ=255)                                                                     cTGTCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCAc            <  1:269648/90‑1 (MQ=255)                                                                        tCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCGCGGt         >  1:27783/1‑90 (MQ=255)                                                                        tCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGt         >  1:66065/1‑90 (MQ=255)                                                                        tCCGGCATACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGt         >  2:113624/1‑90 (MQ=255)                                                                               tACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATc  <  1:16310/90‑1 (MQ=255)                                                                               tACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATc  <  1:384110/90‑1 (MQ=255)                                                                               tACTATCCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATc  <  2:352713/90‑1 (MQ=255)                                                                                     |                                                                                    CACGGATGAAGCGGCTGGGAATTGATAAATCGGCATTGATTTAACTGCAAATTGCCGGACAGATCTGCCTGTCCGGCATACTATTCATGAGGTTTTTTCGGACGATATTTTTCCGGCAGTTCTGGCACCGGACGCTTGTCATCGATGAGATGACGCACGGTTAAGATC  >  NZ_CP009273/2849732‑2849899

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑