Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,995,567	A→G	T635A (ACC→GCC)	xdhA →	xanthine dehydrogenase molybdenum‑binding subunit XdhA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,995,567	0	A	G	100.0%	70.0 / NA	21	T635A (ACC→GCC)	xdhA	xanthine dehydrogenase molybdenum‑binding subunit XdhA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (11/10);  total (11/10)

CTCCATCAAAACCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACACGGCGG  >  NZ_CP009273/2995485‑2995646                                                                                   |                                                                                cTCCATCAAAACCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCaa                                                                          >  2:249339/1‑90 (MQ=255)   ccATCAAAACCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAAcc                                                                        >  1:221976/1‑90 (MQ=255)     aTCAAAACCACCACTAACCCCCCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGc                                                                      <  1:284792/90‑1 (MQ=255)               ccacTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACg                                                            >  2:327110/1‑90 (MQ=255)                          ccGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCa                                                 <  2:242999/90‑1 (MQ=255)                          ccGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCa                                                 <  2:131236/90‑1 (MQ=255)                                tttGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCat                                           <  1:421443/90‑1 (MQ=255)                                  tGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCatat                                         >  1:376512/1‑90 (MQ=255)                                             ttgttgATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCAc                              <  2:114291/90‑1 (MQ=255)                                                 tgATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGCTCATGATTCAGGGCATATTCTTAATCCActgct                          >  2:259992/1‑90 (MQ=255)                                                  gATCTGACGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCActgctg                         <  1:467375/90‑1 (MQ=255)                                                         cGGTCGATATTGCGCTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAg                  >  2:200072/1‑90 (MQ=255)                                                                    gcgcTGTGCAAAGTCGCCATCAACCGCATCCTCAACGCTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTacac       <  1:259992/90‑1 (MQ=255)                                                                       cTGTGCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACAcggc    <  1:389293/90‑1 (MQ=255)                                                                         gtgCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAgg                 <  2:442119/75‑1 (MQ=255)                                                                         gtgCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAgg                 >  1:442119/1‑75 (MQ=255)                                                                         gtgCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACAcggcgg  >  2:253731/1‑90 (MQ=255)                                                                         gtgCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACAcggcgg  >  1:246299/1‑90 (MQ=255)                                                                         gtgCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACAcggcgg  >  1:140716/1‑90 (MQ=255)                                                                         gtgCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACAcg      <  2:119030/86‑1 (MQ=255)                                                                         gtgCAAAGTCGCCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACAcg      >  1:119030/1‑86 (MQ=255)                                                                                   |                                                                                CTCCATCAAAACCACCACTAACCCACCGGCGTTTGGCTGTACCTTTGTTGATCTGACGGTCGATATTGCGCTGTGCAAAGTCACCATCAACCGCATCCTCAACGTTCATGATTCAGGGCATATTCTTAATCCACTGCTGGCAGAAGGTCAGGTACACGGCGG  >  NZ_CP009273/2995485‑2995646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑