Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,179,271	G→A	A167T (GCC→ACC)	ygiL →	fimbrial‑like protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,179,271	0	G	A	100.0%	52.7 / NA	16	A167T (GCC→ACC)	ygiL	fimbrial‑like protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (8/8);  total (8/8)

GTAGTAATTACGTTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCC  >  NZ_CP009273/3179193‑3179345                                                                               |                                                                           gtagtaATTACGTTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGc                                                                 >  1:373850/1‑90 (MQ=255)      aaTTACGTTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGt                                                            >  1:398412/1‑90 (MQ=255)      aaTTACGTTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGt                                                            >  2:301245/1‑90 (MQ=255)         tACGTTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTa                                                         <  1:211436/90‑1 (MQ=255)                    aTACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTg                                              <  2:215373/90‑1 (MQ=255)                                aTCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTg                                  >  2:13764/1‑90 (MQ=255)                                       aCTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGc                                                <  1:52233/69‑1 (MQ=255)                                       aCTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGc                                                >  2:52233/1‑69 (MQ=255)                                       aCTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGa                           >  1:181065/1‑90 (MQ=255)                                             tttCAAAGCTCGTATGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATc                     <  1:200101/90‑1 (MQ=255)                                                           tGGAATCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGt       <  2:137213/90‑1 (MQ=255)                                                              aaTCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGc                                                <  1:427865/46‑1 (MQ=255)                                                              aaTCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGc                                                >  2:427865/1‑46 (MQ=255)                                                                tCCCCAAGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGcc  <  1:301245/90‑1 (MQ=255)                                                                     aaGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTg                                              <  1:3203/41‑1 (MQ=255)                                                                     aaGCAAAGATACCACCCCGGGCAACGTTTACGCTCAGGCTg                                              >  2:3203/1‑41 (MQ=255)                                                                               |                                                                           GTAGTAATTACGTTCAACAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCC  >  NZ_CP009273/3179193‑3179345

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑