Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,771,044	A→G	K96E (AAA→GAA)	lldP →	L‑lactate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,771,044	0	A	G	100.0%	76.7 / NA	23	K96E (AAA→GAA)	lldP	L‑lactate permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/8);  total (15/8)

TGGTTTATGGTTTCTTCTACGGGTTGTGGCCCATCGCGTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGAAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGTCTGCAAATGCTGATCGTCGGTTTC  >  NZ_CP009273/3770964‑3771127                                                                                 |                                                                                    tggtTTATGGTTTCTTCTACGGGTTGTGGCCCATCGCGTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGc                                                                            <  2:169169/90‑1 (MQ=255)             tcttctACGGGTTGTGGCTCATCGCGTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGAcatca                                                                >  1:120611/1‑90 (MQ=255)                      ggTTGTGGCCCATCGCGTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGt                                                       >  1:350721/1‑90 (MQ=255)                                 aTCGCGTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCg                                            >  2:384518/1‑90 (MQ=255)                                  tCGCGTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGc                                                           >  2:368079/1‑74 (MQ=255)                                  tCGCGTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGc                                                           <  1:368079/74‑1 (MQ=255)                                    gcgTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGGGAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATa                                         <  1:286919/90‑1 (MQ=255)                                    gcgTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATa                                         <  2:74877/90‑1 (MQ=255)                                    gcgTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATa                                         <  2:107405/90‑1 (MQ=255)                                    gcgTGGATCATTATTGCAGCGGTGTTCATCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATa                                         <  1:286921/90‑1 (MQ=255)                                              ttattGCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGAcc                               >  1:233679/1‑90 (MQ=255)                                                   gCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGt                          >  2:326866/1‑90 (MQ=255)                                                   gCAGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGt                          >  1:274406/1‑90 (MQ=255)                                                     aGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGTCt                        >  2:238928/1‑90 (MQ=255)                                                     aGCGGTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGTCt                        >  2:264111/1‑90 (MQ=255)                                                        ggTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATa                                         <  1:41139/70‑1 (MQ=255)                                                        ggTGTTCGTCTATAAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATa                                         >  2:41139/1‑70 (MQ=255)                                                                   ataAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGTCTGCAAATGCTGAtcg          >  1:76123/1‑90 (MQ=255)                                                                   ataAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGTCTGCAAATGCTGAtcg          >  2:271076/1‑90 (MQ=255)                                                                    taAGATCTCGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGTCTGCAAATGCTGAtcgt         <  2:203436/90‑1 (MQ=255)                                                                          ctcGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGTCTGCAAATGCTGATCGTCGGttt   >  2:431206/1‑90 (MQ=255)                                                                           tcGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGTCTGCAAATGCTGATCGTCGGTTTc  >  2:142331/1‑90 (MQ=255)                                                                           tcGGTGGAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGTCTGCAAATGCTGATCGTCGGTTTc  >  2:426549/1‑90 (MQ=255)                                                                                 |                                                                                    TGGTTTATGGTTTCTTCTACGGGTTGTGGCCCATCGCGTGGATCATTATTGCAGCGGTGTTCGTCTATAAGATCTCGGTGAAAACCGGGCAGTTTGACATCATTCGCTCGTCTATTCTTTCGATAACCCCTGACCAGCGTCTGCAAATGCTGATCGTCGGTTTC  >  NZ_CP009273/3770964‑3771127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑