| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 385,080 | A→C | I35S (ATC→AGC) | hemB ← | porphobilinogen synthase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 385,080 | 0 | A | C | 100.0% | 44.5 / NA | 15 | I35S (ATC→AGC) | hemB | porphobilinogen synthase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base C (6/9); total (6/9) | |||||||||||
TCGCGTGCCAGATGTTTCTCTGGAATGCGCATCACGCCTGGCATGGCTTCAACGGCTTTGTAGTCGTCAATTTCTTCTTCAACAAAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGG > NZ_CP009273/384993‑385162 | tCGCGTGCCAGATGTTTCTCTGGAATGCGCATCACGCCTGGCATGGCTTCAACGGCTTTGTAGTCGTCAATTTCTTCTTCAACAAAGCTc > 1:79723/1‑90 (MQ=255) gcgTGCCAGATGTTTCTCTGGAATGCGCATCACGCCTGGCATGGCTTCAACGGCTTTGTAGTCGTCAATTTCTTCTTCAACAAAGCTCgg > 2:191970/1‑90 (MQ=255) ctGGAATGCGCATCACGCCTGGCATGGCTTCAACGGCTTTGTAGGCGTCAATTTCTCCTCCAACAAAGCTCGGCAACACCAGGTCGTTaa < 2:263993/90‑1 (MQ=255) ccTGGCATGGCTTCAACGGCTTTGTAGTCGTCAATTTCTTCTTCAACAAAGCTCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTctct < 2:79723/90‑1 (MQ=255) gCTTTGTAGTCGTCAATTTCTTCTTCAACAAAGCTCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCgcgcagc < 2:154473/90‑1 (MQ=255) gTAGTCGTCAATTTCTTCTTCAACAAAGCTCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAgg < 1:163293/90‑1 (MQ=255) gTAGTCGTCAATTTCTTCTTCAACAAAGCTCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAgg < 2:163705/90‑1 (MQ=255) gTAGTCGTCAATTTCTTCTTCAACAAAGCTCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAgg < 2:38319/90‑1 (MQ=255) gTAGTCGTCAATTTCTTCTTCAACAAAGCTCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAgg < 2:428292/90‑1 (MQ=255) cgtcAATTTCTTCTTCAACAAAGCTCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGAtt > 1:361501/1‑90 (MQ=255) cgtcAATTTCTTCTTCAACAAAGCTCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGAtt > 2:210080/1‑90 (MQ=255) ttcttcttcAACAAAGCTCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAg < 2:379628/90‑1 (MQ=255) caacaaAGCTCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAg > 1:38152/1‑90 (MQ=255) caacaaAGCTCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAg < 2:374133/90‑1 (MQ=255) aacaaAGCTCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAgg > 1:38153/1‑90 (MQ=255) | TCGCGTGCCAGATGTTTCTCTGGAATGCGCATCACGCCTGGCATGGCTTCAACGGCTTTGTAGTCGTCAATTTCTTCTTCAACAAAGATCGGCAACACCAGGTCGTTAAGGCTAAGTGTTGTCTCTTCAAACATAGCGCGCAGCGCAGGAGATTTGCGCAGGCGACGAGG > NZ_CP009273/384993‑385162 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |