Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,840,691	T→G	N432H (AAC→CAC)	uhpC ←	MFS transporter family glucose‑6‑phosphate receptor UhpC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,840,691	0	T	G	100.0%	31.4 / NA	11	N432H (AAC→CAC)	uhpC	MFS transporter family glucose‑6‑phosphate receptor UhpC
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (3/8);  total (3/8)

CCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAG  >  NZ_CP009273/3840604‑3840767                                                                                        |                                                                             ccAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAGGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTGCa                                                                            >  1:57804/1‑90 (MQ=255)     gCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTGCaaaaa                                                                        <  1:428730/90‑1 (MQ=255)          aCCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTGCAAAAAGGGCa                                                                   <  1:338601/90‑1 (MQ=255)               gAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTGCAAAAAGGGCAGTAAc                                                              <  1:32629/90‑1 (MQ=255)                                      tatGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTGCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCggcggc                                       <  2:289979/90‑1 (MQ=255)                                              gAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTGCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAgaga                               >  1:418718/1‑90 (MQ=255)                                                         aTGCATCACGCTTCGCGCGGTGTCTGGGCGTGCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACaaaa                    <  2:277939/90‑1 (MQ=255)                                                                   cTTCGCGCGGTGTCTGGGCGTGCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTcc          <  1:109943/90‑1 (MQ=255)                                                                   cTTCGCGCGGTGTCTGGGCGTGCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTcc          <  2:418718/90‑1 (MQ=255)                                                                     tCGCGCGGTGTCTGGGCGTGCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAg        <  1:154826/90‑1 (MQ=255)                                                                           ggTGTCTGGGCGTGCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCCCAAAAAATCCGCTCCAGTGCCAg  >  2:275733/1‑90 (MQ=255)                                                                                        |                                                                             CCAGGCAAAACCTGGAAAATTTCTTAGTTTTGCCGGATATGAAGTGAAAAGGTGAGATGCATCACGCTTCGCGCGGTGTCTGGGCGTTCAAAAAGGGCAGTAACAGCAGTGCGGAAATCCCGGCGGCGATAGAGATAACCACAAAAAATCCGCTCCAGTGCCAG  >  NZ_CP009273/3840604‑3840767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑