Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	444,519	G→A	R527C (CGT→TGT)	cyoB ←	cytochrome o ubiquinol oxidase subunit I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	444,519	0	G	A	100.0%	106.7 / NA	29	R527C (CGT→TGT)	cyoB	cytochrome o ubiquinol oxidase subunit I
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/17);  total (12/17)

CTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGC  >  NZ_CP009273/444435‑444603                                                                                     |                                                                                     cTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGGCGCCAGTCAGGTCACAGTTCt                                                                                 >  1:417397/1‑90 (MQ=255)   aCGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACAGTTCTgg                                                                               >  1:82611/1‑90 (MQ=255)         aaGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACAGTTCTggtcgcgg                                                                         <  1:55914/90‑1 (MQ=255)                    cGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAATAGa                                                              <  2:159466/90‑1 (MQ=255)                     ggAGGCGGGGAAGAGGTTGCCCACTCCAGCGCACGGCCACCCCACGGGGCGCCAGTCAGGGCACAGTTCTGGTCGCGGACGCGAATAGaa                                                             >  1:407593/1‑90 (MQ=255)                      gAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCCCCCCACGGGTCGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAATAGaaa                                                            <  1:57786/90‑1 (MQ=255)                      gAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAATAGaaa                                                            >  1:67079/1‑90 (MQ=255)                         gCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAATAGAAACGt                                                         <  1:88513/90‑1 (MQ=255)                            gggAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAATAGAAACGTACa                                                      <  2:19666/90‑1 (MQ=255)                              gAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAATAGAAACGTACATc                                                    <  1:265565/90‑1 (MQ=255)                                  agGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAATAGAAACGTACATc                                                    <  2:258577/86‑1 (MQ=255)                                  agGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAATAGAAACGTACATc                                                    >  1:258577/1‑86 (MQ=255)                                           aCTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGc                                       >  1:90676/1‑90 (MQ=255)                                                 gCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGaa                                 <  1:89082/90‑1 (MQ=255)                                                      cGGCCACCCCACGGGTCGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATAccc                            >  1:363447/1‑90 (MQ=255)                                                          cACCCCACGGGTCGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAgcg                        <  1:448925/90‑1 (MQ=255)                                                          cACCCCACGGGTCGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAgcg                        <  2:86399/90‑1 (MQ=255)                                                          cACCCCACGGGTCGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAgcg                        <  1:362682/90‑1 (MQ=255)                                                          cACCCCACGGGTCGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAgcg                        <  1:337748/90‑1 (MQ=255)                                                             cccACGGGTCGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCaa                     <  1:172465/90‑1 (MQ=255)                                                               cACGGGTCGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATc                   <  1:301871/90‑1 (MQ=255)                                                                aCGGGTCGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATc                   >  2:210156/1‑89 (MQ=255)                                                                aCGGGTCGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATc                   <  1:210156/89‑1 (MQ=255)                                                                      cGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAAt                                                                 >  1:243320/1‑37 (MQ=255)                                                                      cGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAAt                                                                 <  2:243320/37‑1 (MQ=255)                                                                      cGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTg            >  1:146072/1‑90 (MQ=255)                                                                      cGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTg            >  1:253268/1‑90 (MQ=255)                                                                      cGCCAGTCAGGTCACAGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTg            >  2:191361/1‑90 (MQ=255)                                                                                gTCACAGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCgctggc  <  1:402873/90‑1 (MQ=255)                                                                                     |                                                                                     CTACGGCAAAGTTATAGAACGGAGGCGGGGAAGAGGTTGCCCACTCCAGCGTACGGCCACCCCACGGGTCGCCAGTCAGGTCACGGTTCTGGTCGCGGTCGCGAATAGAAACGTACATCTGAATAACGAGGCAGAGAATACCCAGCGCAATCAGTACTGCACCGCTGGC  >  NZ_CP009273/444435‑444603

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑