Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 4,306,692 A→T I115N (ATC→AAC)  phnM ← alpha‑D‑ribose 1‑methylphosphonate 5‑triphosphate diphosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,306,6920AT92.3% 36.6 / ‑3.3 13I115N (ATC→AAC) phnMalpha‑D‑ribose 1‑methylphosphonate 5‑triphosphate diphosphatase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (5/7);  total (5/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.91e-01

ATGCGGCAGTTCGCAGCGCAGATGCAGACGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTACGGTGGTGAT  >  NZ_CP009273/4306613‑4306771
                                                                               |                                                                               
aTGCGGCAGTTCGCAGCGCAGATGCAGACGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGTTCATCTtctc                                                                       >  1:248562/1‑90 (MQ=255)
  gCGGCAGTTCGCAGCGCAGATGCAGACGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGGTGTTCATCTTCTCCa                                                                     >  2:314953/1‑90 (MQ=255)
   cGGCAGTTCGCAGCGCAGATGCAGACGGTGCTCGGCGCGGTTGACGCCGCGTTCCTGCGTCTCTTCGATGGCGTTGTTCATCTTCTCCAg                                                                    <  2:335542/90‑1 (MQ=255)
                  cagATGCAGACGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGTTCATCTTCTCCAGATTCTCCAGCCGATc                                                     <  2:248562/90‑1 (MQ=255)
                            cGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTTTTCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTcc                                           >  2:384915/1‑89 (MQ=255)
                                        cgGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGTTCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCa                               >  1:79581/1‑90 (MQ=255)
                                                  cgcgTTTCTGCGTCTCTTCGATGGCGTTGTTCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACgg                     <  1:143396/90‑1 (MQ=255)
                                                  cgcgTTTCTGCGTCTCTTCGATGGCGTTGTTCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACgg                     <  2:131212/90‑1 (MQ=255)
                                                    cgTTTCTGCGTCTCTTCGATGGCGTTGTTCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCa                   >  1:144739/1‑90 (MQ=255)
                                                    cgTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCa                   <  2:201074/90‑1 (MQ=255)
                                                       ttCTGCGTCTCTTCGATGGCGTTGTTCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATcc                <  2:41487/90‑1 (MQ=255)
                                                          tGCGTCTCTTCGATGGCGTTGTTCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGt             <  1:15500/90‑1 (MQ=255)
                                                                     gATGGCGTTGTTCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTACGGTGGTGAt  <  2:79581/90‑1 (MQ=255)
                                                                               |                                                                               
ATGCGGCAGTTCGCAGCGCAGATGCAGACGGTGCTCGGCGCGGTTGACGCCGCGTTTCTGCGTCTCTTCGATGGCGTTGATCATCTTCTCCAGATTCTCCAGCCGATCGCCGCCGTCGCGCACGTCGCCAATTGCCACGGCATCCAGTACGGTGGTGAT  >  NZ_CP009273/4306613‑4306771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑