Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	885,317	T→C	intergenic (‑42/‑228)	ybjL ← / → ybjM	aspartate:alanine antiporter/inner membrane protein YbjM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	885,317	0	T	C	100.0%	28.2 / NA	10	intergenic (‑42/‑228)	ybjL/ybjM	aspartate:alanine antiporter/inner membrane protein YbjM
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/6);  total (4/6)

GAATGTAATTCCCATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAACGATAGTAAAG  >  NZ_CP009273/885233‑885390                                                                                     |                                                                          gAATGTAATTCCCATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAACGGTaa                                                                      >  1:438132/1‑90 (MQ=255)             cATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAACGGTAATTTACGATAATg                                                          >  1:302193/1‑90 (MQ=255)                                    atatTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAACGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGgcgc                                   <  2:303662/90‑1 (MQ=255)                                    atatTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAACGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGgcgc                                   <  2:317978/90‑1 (MQ=255)                                    atatTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAACGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGgcgc                                   <  2:418310/90‑1 (MQ=255)                                                  tAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAACGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAgcg                     >  1:165593/1‑90 (MQ=255)                                                           gTTTACTAGTAAGCTGTTGAAAGAAACGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAAc            <  1:76983/90‑1 (MQ=255)                                                           gTTTACTAGTAAGCTGTTGAAAGAAACGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAAc            <  2:213000/90‑1 (MQ=255)                                                               aCTAGTAAGCTGTTGAAAGAAACGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAACGATa        <  2:327339/90‑1 (MQ=255)                                                                     aaGCTGTTGAAAGAAACGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAACGATagtaaag  >  1:202858/1‑90 (MQ=255)                                                                                     |                                                                          GAATGTAATTCCCATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAACGATAGTAAAG  >  NZ_CP009273/885233‑885390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑