Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	991,969	T→G	L144F (TTA→TTC)	ssuA ←	aliphatic sulfonate ABC transporter substrate‑binding protein SsuA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	991,969	0	T	G	100.0%	27.7 / NA	10	L144F (TTA→TTC)	ssuA	aliphatic sulfonate ABC transporter substrate‑binding protein SsuA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/3);  total (7/3)

GCGGCGCGGGCATCAGCGGGCGTCAGGTAAGTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTC  >  NZ_CP009273/991889‑992052                                                                                 |                                                                                    gcggcgcggGCATCAGCGGGCGTCAGGTAAGTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGGAAAAGGTtg                                                                            >  1:304104/1‑90 (MQ=255)                   ggCGTCAGGTAAGTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCGGGAAAAGGGTGTGTGAACTGGAACCTTTc                                                          >  2:192371/1‑90 (MQ=255)                             aaGTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCaa                                                >  1:265076/1‑90 (MQ=255)                                     tGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGa                                        <  2:265697/90‑1 (MQ=255)                                           tCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTa                                  >  2:246224/1‑90 (MQ=255)                                                            ggCCTGACGCAGTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGtttt                 >  2:363373/1‑90 (MQ=255)                                                             gCCTGACGCAGTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTg                >  1:78599/1‑90 (MQ=255)                                                               cTGACGCAGTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGAt              <  2:175096/90‑1 (MQ=255)                                                                    gCAGTGCACGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGc         <  1:224393/90‑1 (MQ=255)                                                                           aCGCAGGAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTc  >  1:147082/1‑90 (MQ=255)                                                                                 |                                                                                    GCGGCGCGGGCATCAGCGGGCGTCAGGTAAGTGGGTTGGATATCGGTAAACTTAAGTCCGGCCTGACGCAGTGCACGCAGTAAAAGGTTGTGTGAACTGGAACCTTTCTGAAAGGCAACTTTGTGACCTTTAAGATCGGCTACGGTTTTGATCGGGCTGTTTTC  >  NZ_CP009273/991889‑992052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑