Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,155,022	G→T	T662T (ACC→ACA)	fhuE ←	ferric‑rhodotorulic acid/ferric‑coprogen receptor FhuE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,155,022	0	G	T	100.0%	30.0 / NA	9	T662T (ACC→ACA)	fhuE	ferric‑rhodotorulic acid/ferric‑coprogen receptor FhuE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (3/6);  total (3/6)

GAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGC  >  NZ_CP009273/1154935‑1155088                                                                                        |                                                                   gaAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACTgt                                                                  <  1:155651/90‑1 (MQ=255)      ttttCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACTGTGTCgg                                                             <  2:245239/90‑1 (MQ=255)              aCCTGGTAGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACTGTGTCGGTATACAcg                                                     <  1:263884/90‑1 (MQ=255)              aCCTGGTAGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACTGTGTCGGTATACAcg                                                     <  1:279601/90‑1 (MQ=255)                                 gaTCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACTGTGTCGGTATACACGCGATTTTGCCAGTTAacac                                  >  1:314935/1‑90 (MQ=255)                                         aGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACTGTGTCGGTATACACGCGATTTTGCCAGTTAACACCAccgccg                          <  1:104229/90‑1 (MQ=255)                                                         gCTCGGCGCGGAAGGTGCCATACGGCGTCACTGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCa          >  1:271380/1‑90 (MQ=255)                                                              gcgcGGAAGGTGCCATACGGCGTCACTGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACa     >  1:39657/1‑90 (MQ=255)                                                                 cgGAAGGTGCCATACGGCGTCACTGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGc  <  1:179466/90‑1 (MQ=255)                                                                                        |                                                                   GAAGTTTTTCGTCACCTGGTAGCGGGTGAAGAGATCCACCAGCGCGTAGCTACCTTGCTCGGCGCGGAAGGTGCCATACGGCGTCACGGTGTCGGTATACACGCGATTTTGCCAGTTAACACCACCGCCGACCGTCAACTCTGGCATGACAGGC  >  NZ_CP009273/1154935‑1155088

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑