Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,365,912	T→G	R480R (CGT→CGG)	ycjM →	glucosylglycerate phosphorylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,365,912	0	T	G	100.0%	43.6 / NA	14	R480R (CGT→CGG)	ycjM	glucosylglycerate phosphorylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/6);  total (8/6)

AGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCG  >  NZ_CP009273/1365826‑1365980                                                                                       |                                                                     aGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGgcgc                                                                   <  1:272845/90‑1 (MQ=255)            aaCCCGAGAACTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGGCGCAGCCATAACg                                                         <  1:118091/89‑1 (MQ=255)            aaCCCGAGAACTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGGCGCAGCCATAACg                                                         <  1:140509/89‑1 (MQ=255)            aaCCCGAGAACTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGGCGCAGCCATAACg                                                         >  2:118091/1‑89 (MQ=255)            aaCCCGAGAACTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGGCGCAGCCATAACg                                                         >  2:140509/1‑89 (MQ=255)            aaCCCGAGAACTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGGCGCAGCCATAACGa                                                        >  2:278168/1‑90 (MQ=255)                        gAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGGCGCAGCCATAACGAGTTTCATCCGGa                                            >  2:63393/1‑90 (MQ=255)                                          aaGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGGCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGa                          >  2:5510/1‑90 (MQ=255)                                            ggCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGGCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATa                        >  1:272159/1‑90 (MQ=255)                                               aTGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGGCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGa                     >  2:295660/1‑90 (MQ=255)                                                 gCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGGCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGAtt                   <  1:235648/90‑1 (MQ=255)                                                      atatCATGAGTTGTCGCGTTTAATTACACTTCGGCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTc              <  2:38642/90‑1 (MQ=255)                                                         tCATGAGTTGTCGCGTTTAATTACACTTCGGCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATc           <  1:63393/90‑1 (MQ=255)                                                             gAGTTGTCGCGTTTAATTACACTTCGGCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTa       <  2:182884/90‑1 (MQ=255)                                                                  gTCGCGTTTAATTACACTTCGGCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCg  >  2:274658/1‑90 (MQ=255)                                                                                       |                                                                     AGTAAAGAGATAACCCGAGAACTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGTAATGCG  >  NZ_CP009273/1365826‑1365980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑