Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,394,163	G→A	L111L (CTG→TTG)	ogt ←	methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,394,163	0	G	A	100.0%	29.0 / NA	9	L111L (CTG→TTG)	ogt	methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/3);  total (6/3)

ATGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCGTAGCA  >  NZ_CP009273/1394090‑1394248                                                                          |                                                                                      aTGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCAtt                                                                       >  2:264715/1‑90 (MQ=255)          ggATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCg                                                              <  1:5448/90‑1 (MQ=255)            aTTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCa                                                            <  1:51534/90‑1 (MQ=255)                               ccAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGtt                                         >  2:183009/1‑90 (MQ=255)                                       aCGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGAc                                 >  2:50792/1‑90 (MQ=255)                                                         gCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCgcgc                          >  1:410568/1‑79 (MQ=255)                                                         gCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCgcgc                          <  2:410568/79‑1 (MQ=255)                                                         gCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGt               >  1:435784/1‑90 (MQ=255)                                                                      gCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCGTAGCa  >  1:210917/1‑90 (MQ=255)                                                                          |                                                                                      ATGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCGTAGCA  >  NZ_CP009273/1394090‑1394248

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑