Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,394,663	T→C	G456G (GGA→GGG)	abgT ←	p‑aminobenzoyl‑glutamate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,394,663	0	T	C	100.0%	29.6 / NA	10	G456G (GGA→GGG)	abgT	p‑aminobenzoyl‑glutamate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/3);  total (7/3)

AAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGATTTGCG  >  NZ_CP009273/1394587‑1394742                                                                             |                                                                                aaaaGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTg                                                                    <  1:264715/90‑1 (MQ=255) aaaaGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTg                                                                    <  2:106034/90‑1 (MQ=255)                           aaCGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTaaa                                          >  1:326706/1‑90 (MQ=255)                                       gtaCCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGAt                              >  1:397382/1‑90 (MQ=255)                                                   gcgTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATa                  >  2:221955/1‑90 (MQ=255)                                                                   gTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGAttt     <  1:448654/87‑1 (MQ=255)                                                                   gTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGAttt     >  2:448654/1‑87 (MQ=255)                                                                   gTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGATTTgcg  >  2:25817/1‑90 (MQ=255)                                                                   gTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGATTTgcg  >  2:57191/1‑90 (MQ=255)                                                                   gTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGATTTgcg  >  2:93857/1‑90 (MQ=255)                                                                             |                                                                                AAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGATTTGCG  >  NZ_CP009273/1394587‑1394742

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑