| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 130,698 | A→T | *866L (TAA→TTA) | acnB → | bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 130,698 | 0 | A | T | 100.0% | 83.3 / NA | 22 | *866L (TAA→TTA) | acnB | bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase |
| Reads supporting (aligned to +/- strand): ref base A (0/0); new base T (12/10); total (12/10) | |||||||||||
ATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATC > NZ_CP009273/130617‑130781 | aTACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCa < 1:140772/90‑1 (MQ=255)aTACTTACCGTTATCTGAACTTCAACCAGCTTCCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCa < 1:260186/90‑1 (MQ=255) ttACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAgcgc > 1:128025/1‑90 (MQ=255) aCCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCAc > 1:360802/1‑90 (MQ=255) ttATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACgcgc < 1:235702/90‑1 (MQ=255) ttATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACgcgc > 1:260016/1‑90 (MQ=255) ttATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACgcgc < 2:128025/90‑1 (MQ=255) tATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCt < 1:293965/90‑1 (MQ=255) tATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCt < 2:450938/90‑1 (MQ=255) aTCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTg > 1:138348/1‑90 (MQ=255) gAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCa > 2:6827/1‑90 (MQ=255) tCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAAc < 1:6827/90‑1 (MQ=255) tCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAAc > 2:635/1‑90 (MQ=255) cAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACg > 2:153262/1‑90 (MQ=255) ccGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATga > 1:321963/1‑90 (MQ=255) ccGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATga > 1:162415/1‑90 (MQ=255) aGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTccc > 1:86758/1‑90 (MQ=255) cGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCggg < 2:124195/90‑1 (MQ=255) aTGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCt > 1:425953/1‑47 (MQ=255) aTGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCt < 2:425953/47‑1 (MQ=255) gATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCAttttttt > 1:461375/1‑90 (MQ=255) cGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATc < 2:251573/90‑1 (MQ=255) | ATACTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCATC > NZ_CP009273/130617‑130781 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |