Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,505,430	A→G	K391E (AAA→GAA)	ydcR →	PLP‑dependent aminotransferase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,505,430	0	A	G	100.0%	29.0 / NA	10	K391E (AAA→GAA)	ydcR	PLP‑dependent aminotransferase family protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/3);  total (7/3)

GCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCG  >  NZ_CP009273/1505360‑1505510                                                                       |                                                                                 gCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAg                                                               >  2:270960/1‑90 (MQ=255)                               tGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGa                                 <  1:104712/90‑1 (MQ=255)                                gCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAg                                >  2:164803/1‑90 (MQ=255)                                 ccTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGc                               <  1:164803/90‑1 (MQ=255)                                              gctgcGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGAc                                                                 >  1:247360/1‑43 (MQ=255)                                              gctgcGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGAc                                                                 <  2:247360/43‑1 (MQ=255)                                              gctgcGTTATCTGCCTGCGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCgg                  >  1:377144/1‑90 (MQ=255)                                                        ctgcctgcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGc        >  1:210930/1‑90 (MQ=255)                                                           cctgcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTg     >  2:327225/1‑90 (MQ=255)                                                              gcGGAAGTGGAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTggcg  >  1:454490/1‑90 (MQ=255)                                                                       |                                                                                 GCGTCGCCAGCTTGCGGAACGTAAACAACGTGCCTGGCAGGCACTGCTGCGTTATCTGCCTGCGGAAGTGAAAATTCATCATAATGACAGTGGTTACTTTCTCTGGTTGGAGCTCCCCGAGCCGTTAGATGCCGGCGAATTAAGCCTGGCG  >  NZ_CP009273/1505360‑1505510

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑