Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,579,109	A→C	G545G (GGT→GGG)	ydeP ←	acid resistance putative oxidoreductase YdeP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,579,109	0	A	C	100.0%	35.9 / NA	12	G545G (GGT→GGG)	ydeP	acid resistance putative oxidoreductase YdeP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (6/6);  total (6/6)

CAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACACCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGTTTTTT  >  NZ_CP009273/1579025‑1579190                                                                                     |                                                                                  caGATACTCCCAGGCTACCACCCTTTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCcggc                                                                              <  2:369867/90‑1 (MQ=255)                tACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAAcacgcc                                                               <  2:125829/90‑1 (MQ=255)                       ctctGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAg                                                        >  2:447785/1‑90 (MQ=255)                         ctGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGc                                                      >  1:300060/1‑90 (MQ=255)                                 gTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAatca                                              >  1:301576/1‑90 (MQ=255)                                     tgcctgcGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGa                                          <  2:63813/90‑1 (MQ=255)                                            gcgATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGaa                                   <  1:410127/90‑1 (MQ=255)                                                  cccGCGACCACTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCa                             <  2:301576/90‑1 (MQ=255)                                                           aCTGCACACTCTGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTAcc                    <  2:182799/90‑1 (MQ=255)                                                                   ctctGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTgcgc            >  1:153598/1‑90 (MQ=255)                                                                   ctctGATTTCAGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTgcgc            >  1:432422/1‑90 (MQ=255)                                                                             aGCATTACCCCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGtttttt  >  1:95833/1‑90 (MQ=255)                                                                                     |                                                                                  CAGATACTCCCAGGCTACCACGCTCTGGGGTAGTGCTGCCTGCGCGATTCCCGCGACCACTGCACACTCTGATTTCAGCATTACACCGGCGGGTTTTAACACGCCACGCGAGGCATGAATCATCGACATTGAATCCTCAACGGTTACCGCCTGCGCACCGTTTTTT  >  NZ_CP009273/1579025‑1579190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑