Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,797,377	(T)5→4	coding (517/960 nt)	nrdF →	class 1b ribonucleoside‑diphosphate reductase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,797,373	0	T	.	100.0%	120.4 / NA	29	coding (513/960 nt)	nrdF	class 1b ribonucleoside‑diphosphate reductase subunit beta
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (6/23);  total (6/23)

GGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACAT  >  NZ_CP009273/2797290‑2797456                                                                                    |                                                                                    ggTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTAttttcca                                                                               >  1:134229/1‑87 (MQ=255) ggTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTAttttcca                                                                               >  2:409850/1‑87 (MQ=255)    gatgatCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTCTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTA‑TTTTCCAGcc                                                                           >  1:383137/1‑90 (MQ=255)       gatCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTA‑TTTTCCAGCcgcg                                                                        <  2:569808/90‑1 (MQ=255)        atCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTA‑TTTTCCAGCCGCgg                                                                       <  1:209385/90‑1 (MQ=255)            gCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTA‑TTTTCCAGCCGCGGAAAg                                                                   >  1:517396/1‑90 (MQ=255)             cTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTA‑TTTTCCAGCCGCGGAAAGc                                                                  <  1:263482/90‑1 (MQ=255)             cTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTA‑TTTTCCAGCCGCGGAAAGc                                                                  <  1:442528/90‑1 (MQ=255)              tGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTA‑TTTTCCAGCCGCGGAAAGc                                                                  <  2:251250/89‑1 (MQ=255)              tGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTA‑TTTTCCAGCCGCGGAAAGc                                                                  >  1:251250/1‑89 (MQ=255)              tGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTA‑TTTTCCAGCCGCGGAAAGCt                                                                 <  1:656584/90‑1 (MQ=255)                          tCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTA‑TTTTCCAGCCGCGGAAAGCTGACCAATACcgc                                                     <  1:464754/90‑1 (MQ=255)                               aGTGTGTTTCTTGAATCTTTTTTGTTATATTCCGGTTTCTGGCTGCCGATGTA‑TTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGAcc                                                <  2:327757/90‑1 (MQ=255)                                   tgtTTCTTGAATCTCTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTA‑TTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGAt                                            <  2:383137/90‑1 (MQ=255)                                    gtTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTA‑TTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATc                                           >  1:398863/1‑90 (MQ=255)                                       tCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTA‑TTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGt                                        >  1:534597/1‑90 (MQ=255)                                        cTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTA‑TTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTc                                       <  1:646678/90‑1 (MQ=255)                                        cTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTA‑TTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTc                                       <  2:216765/90‑1 (MQ=255)                                                          tATTCCGGTTTCTGGCTGCCGATGTA‑TTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAg                     <  1:327764/90‑1 (MQ=255)                                                           aTTCCGGTTTCTGGCTGCCGATGTA‑TTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGc                    <  2:412055/90‑1 (MQ=255)                                                           aTTCCGGTTTCTGGCTGCCGATGTA‑TTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGc                    <  2:391852/90‑1 (MQ=255)                                                                  tttCTGGCTGCCGATGTA‑TTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCAc             <  1:191004/90‑1 (MQ=255)                                                                  tttCTGGCTGCCGATGTA‑TTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCAc             <  1:403929/90‑1 (MQ=255)                                                                  tttCTGGCTCCCGATGTA‑TTTTCCAGCCGCGGAAAGCTGCCCAATCCCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTcccc             <  2:371266/90‑3 (MQ=255)                                                                   ttCTGGCTGCCGATGTA‑TTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACg            <  2:137448/90‑1 (MQ=255)                                                                   ttCTGGCTGCCGATGTA‑TTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACg            <  1:650488/90‑1 (MQ=255)                                                                     cTGGCTGCCGATGTA‑TTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGt          >  1:432926/1‑90 (MQ=255)                                                                        gCTGCCGATGTA‑TTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTtac       <  2:534597/90‑1 (MQ=255)                                                                             cGATGTA‑TTTTCCAGCCGCGGTAAGCTGACCAATACCACGGACCGGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACAt  <  1:569949/90‑1 (MQ=255)                                                                             cGATGTA‑TTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACAt  <  2:262688/90‑1 (MQ=255)                                                                             cGATGTA‑TTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACAt  <  1:520069/90‑1 (MQ=255)                                                                                    |                                                                                    GGTGATGATCCGCTGAAAAAGAAAATCGCCAGTGTGTTTCTTGAATCTTTTTTGTTCTATTCCGGTTTCTGGCTGCCGATGTATTTTTCCAGCCGCGGAAAGCTGACCAATACCGCGGACCTGATCCGTCTGATTATCCGCGATGAAGCAGTCCACGGTTACTACAT  >  NZ_CP009273/2797290‑2797456

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑