Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	579,943	(T)8→7	intergenic (+57/+193)	appY → / ← ompT	DNA‑binding transcriptional activator AppY/omptin family outer membrane protease OmpT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	579,936	0	T	.	100.0%	23.8 / NA	7	intergenic (+50/+200)	appY/ompT	DNA‑binding transcriptional activator AppY/omptin family outer membrane protease OmpT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (4/3);  total (4/3)

GTTTATTACAAGTTGTCTACATGTTAATTATAATATTATACAGCGTTTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACTATAATTGATGGTT  >  NZ_CP009273/579891‑580018                                              |                                                                                   gTTTATTACAAGTTGTCTACATGTTAATTATAATATTATACAGCG‑TTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGtt                                       <  2:568233/90‑1 (MQ=255)             ttGTCTACATGTTAATTATAATATTATACAGCG‑TTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGCCTAt                           <  1:400856/90‑1 (MQ=255)                                aataTTATACAGCG‑TTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACTATAATTg        >  2:388192/1‑90 (MQ=255)                                   attatACAGCG‑TTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACTATAATTGATg     <  2:492053/90‑1 (MQ=255)                                      ataCAGCG‑TTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACTATAATTGATGGtt  >  1:105285/1‑90 (MQ=255)                                      ataCAGCG‑TTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACTATAATTGATGGtt  >  1:455264/1‑90 (MQ=255)                                      ataCAGCG‑TTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACTATAATTGATGGtt  >  1:524768/1‑90 (MQ=255)                                              |                                                                                   GTTTATTACAAGTTGTCTACATGTTAATTATAATATTATACAGCGTTTTTTTTGATGTGATATTCTGGAACCATTAATTTGTAATTGGGTTGCTGTCGCCTATTTTATACATACTATAATTGATGGTT  >  NZ_CP009273/579891‑580018

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑