Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,300,524	Δ1 bp	coding (504/1005 nt)	oppF →	murein tripeptide/oligopeptide ABC transporter ATP‑binding protein OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,300,522	0	G	.	100.0%	49.6 / NA	13	coding (502/1005 nt)	oppF	murein tripeptide/oligopeptide ABC transporter ATP‑binding protein OppF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (7/6);  total (7/6)

GATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTC  >  NZ_CP009273/1300461‑1300598                                                              |                                                                             gatgCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGt                                                 <  2:408178/90‑1 (MQ=255)  atgCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTg                                                <  2:618208/90‑1 (MQ=255)     cTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGctc                                             >  2:463703/1‑90 (MQ=255)                       ccTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGc                           <  1:586797/90‑1 (MQ=255)                         tAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTg                         >  1:398003/1‑90 (MQ=255)                           aCCTGATTAACCGCTATCCGCAGGAGTTCTCTGGT‑GGCAGAGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTTAACCGAAGCTGAt                       >  1:304185/1‑90 (MQ=255)                                aTTAACCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCt                  >  1:502133/1‑90 (MQ=255)                                    aCCGCTATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGtga              <  2:345423/90‑1 (MQ=255)                                         tATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGc         >  1:194246/1‑90 (MQ=255)                                         tATCCGCATGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGc         >  2:562279/1‑90 (MQ=255)                                                aTGAGTTCTCTGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTc  <  2:278057/90‑1 (MQ=255)                                                         ctGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCtt                                           <  1:445183/40‑1 (MQ=39)                                                         ctGGT‑GGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCtt                                           >  2:445183/1‑40 (MQ=39)                                                              |                                                                             GATGCTGAAAGTCGGGTTATTGCCTAACCTGATTAACCGCTATCCGCATGAGTTCTCTGGTGGGCAGTGCCAGCGTATCGGGATTGCGCGTGCTCTTATTCTTGAACCGAAGCTGATTATCTGTGATGAGCCGGTGTC  >  NZ_CP009273/1300461‑1300598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑