| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 3,934,995 | +C | intergenic (‑308/‑173) | yieP ← / → BW25113_RS19520 | FadR/GntR family transcriptional regulator/16S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,934,991 | 1 | . | C | 96.0% | 75.6 / ‑4.3 | 25 | intergenic (‑304/‑177) | yieP/BW25113_RS19520 | FadR/GntR family transcriptional regulator/16S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base C (13/11); total (14/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTCTCCTGAGAACTCCGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCAC‑A‑CCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAG > NZ_CP009273/3934918‑3935067 | gTTCTCCTGAGAACTCCGGCAGAGAAAGTAACAATAAATGCGTGACTCTGTAGAGGGAGATCGTATTATGCACAA‑CCCCGCGCCGCTgag > 2:565601/1‑90 (MQ=11)gTTCTCCTGAGAACTCCGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCAC‑ACCCCCGCGCCGCTgag > 2:550778/1‑90 (MQ=255) aCTCCGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAaaagcaaagc < 1:145084/90‑1 (MQ=255) cTCCGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCg > 2:162641/1‑90 (MQ=255) ccGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGc > 1:99213/1‑90 (MQ=255) ccGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGc > 2:298261/1‑90 (MQ=255) ccGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGc > 2:266435/1‑90 (MQ=255) gCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACt < 1:421857/90‑1 (MQ=255) gCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACt < 1:117981/90‑1 (MQ=255) agagAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGc > 2:575483/1‑90 (MQ=255) agagAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGc < 1:275285/90‑1 (MQ=255) agagAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGc < 2:530944/90‑1 (MQ=255) aatGCTTGACTCTGTAGCGGGAAAGCGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATc < 2:66139/90‑1 (MQ=255) tGCTTGACTCTGTAGCGGGAAAGCGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAg < 1:378730/90‑1 (MQ=255) cTTGACTCTGTAGCGGGAAAGCGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGAc < 2:87010/90‑1 (MQ=255) aCTCTGTAGCGGGAAAGCGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATc < 2:621152/90‑1 (MQ=255) gggAAAGCGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCa > 2:203783/1‑90 (MQ=255) gggAAAGCGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCa > 2:237255/1‑90 (MQ=255) gggAAAGCGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCa > 1:304386/1‑90 (MQ=255) gggAAAGCGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCa > 2:121006/1‑90 (MQ=255) gggAAAGCGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCa > 1:94985/1‑90 (MQ=255) gggAAAGCGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCa > 1:320891/1‑90 (MQ=255) aGCGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACaa > 2:175453/1‑73 (MQ=255) aGCGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACaa < 1:175453/73‑1 (MQ=255) cGTATTATGCAC‑ACCCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAg < 1:522934/90‑1 (MQ=255) | GTTCTCCTGAGAACTCCGGCAGAGAAAGCAAAAATAAATGCTTGACTCTGTAGCGGGAAAGCGTATTATGCAC‑A‑CCCCGCGCCGCTGAGAAAAAGCAAAGCGGCACTGCTCTTTAACAATTTATCAGACAATCTGTGTGGGCACTCGAAG > NZ_CP009273/3934918‑3935067 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |