| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 228,191 | 0 | A | G | 56.2% | 6.3 / 17.2 | 16 | S195G (AGC→GGC) | yafD | endonuclease/exonuclease/phosphatase family protein |
| Reads supporting (aligned to +/- strand): ref base A (1/6); new base G (9/0); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.98e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGA > NZ_CP009273/228104‑228261 | gTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGc < 2:527029/90‑1 (MQ=255) taGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAg < 1:69289/90‑1 (MQ=255) aaGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTCTGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAgg > 2:27943/1‑90 (MQ=255) aaGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGTTTTAAATCCTGGGGGCGGAGAAgg > 2:124341/1‑90 (MQ=255) aCTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAAcgc < 1:247841/90‑1 (MQ=255) aCTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAAcgc < 2:586968/90‑1 (MQ=255) cTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGTTTTAATGGCTGGGGGCGGGGAAGGAGGAAagcggttt > 2:98882/1‑86 (MQ=255) cTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGGGGCGGAGAAGGGTGAACGCGGtat > 1:244547/1‑90 (MQ=255) tGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGATTTTAATTGCTGGGGCCGGGGAAGGGAGGAAACGTTatattg > 1:424909/1‑88 (MQ=255) gATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGGGGTTTTTATTCCTCGGGGCGCAGGAGGGGGAAAGCGGTTTATTGCttt > 2:279416/1‑90 (MQ=255) aGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTgcgc < 2:514037/90‑1 (MQ=255) gATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGAGTTTAATGCCCTGGGGCGTAGAAGGATGGACGCGGTATATTGCTTTtcggg > 1:531892/1‑88 (MQ=255) caccacAGCGGCCCGGTCATTATGGCGGGAGGTTTCAATGCCTGGGGGCGTAGAAGGATGAAAGCGTTTTATCGCTTTTCGCGGGgaaag > 1:54062/1‑88 (MQ=255) gCGGCCCGGTCATTATGGCGGGGGAGTTTAATGGCTGGGGGCGGAGGAGGAGGAAAGCGGTATATTGCTTTTTGCGGGGAAAGTTGCCgc > 2:199070/1‑90 (MQ=255) gTCATTATGGCGGGAGGGTTTAATTCCTGGGGCCGGAGAAGGGTGGACGGGGTTTATCGCTTTTCGCGGGGAAAGTTGCTGCGCCCgggg > 1:396595/1‑88 (MQ=255) gggAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCga < 1:401531/90‑1 (MQ=255) | GTCTATAGTAAGCAGTTACTTCCTATTGGCGATCAGATAGCTCACCACAGCGGCCCGGTCATTATGGCGGGAGATTTCAATGCCTGGAGCCGTAGAAGGATGAACGCGTTATATCGCTTTGCGCGGGAAATGTCGCTGCGCCAGGTGCGTTTTACCGA > NZ_CP009273/228104‑228261 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |