| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 500,939 | 0 | T | A | 61.9% | 3.6 / 18.0 | 21 | R190R (CGT→CGA) | ushA | bifunctional UDP‑sugar hydrolase/5'‑nucleotidase |
| Reads supporting (aligned to +/- strand): ref base T (6/2); new base A (0/13); total (6/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.16e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.85e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGGATCTGAAAATTGCCGTTATTGGGCTGACAACCGATGACACAGCAAAAATTGGTAACCC‑GG‑AATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATAT > NZ_CP009273/500850‑501022 | cAGGATCTGAAAATTGCCGTTATTGGGCTGACAACCGATGACACAGCAAAAATTGGTAACCC‑GG‑AATACTTCACTGATATCGAATTTCGt > 1:115637/1‑90 (MQ=255)cAGGATCTGAAAATTGCCGTTATTGGGCTGACAACCGATGACACAGCAAAAATTGGTAACCC‑GG‑AATACTTCACTGATATCGAATTTCGt > 2:117307/1‑90 (MQ=255)cAGGATCTGAAAATTGCCGTTATTGGGCTGACAACCGATGACACAGCAAAAATTGGTAACCC‑GG‑AATACTTCACTGATATCGAATTTCGt > 1:6627/1‑90 (MQ=255) ttATTGGGCTGACACCCGATGCCACAGAAAAAATTGGTACCCC‑GA‑AATCCTTCACTGATATCGATTTTCGTAAGCCCGCCGATgaagcga < 1:117307/90‑1 (MQ=255) ttATTGGGCTGACAACCGATGACACAGAAAAAATTGGTAACCC‑GA‑AATACTTCACTGATATCAAATTTCGTAAGCCCGCCGATgaagcga < 1:634900/90‑1 (MQ=255) ttATTGGCCTAAAACCCAATAACAAAAAAAAAATTGGTACCCC‑GA‑AATCCTTCACTAATATCAATTTTCGAAAGCCCGCCGATgaagcga < 2:115637/90‑1 (MQ=255) ttggggCTGACACCCGATGACACA‑CAAAAATTTGGAACCCGGG‑AATCCTTCACTAATTTGGATTTTGGAAGCCCCGCCGATgaagcgaag < 2:178505/87‑1 (MQ=255) gACAACCGATGACACAGCAAAAATTGGTAACCC‑GG‑AATACTTCACTGATATCGAACTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGAt > 1:121463/1‑90 (MQ=255) gATGACACAGCAAAAATTGGTAACCC‑GG‑AATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAg > 2:5622/1‑90 (MQ=255) caGCAAAAATTGGTAACCC‑GG‑AATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAac > 1:465402/1‑90 (MQ=255) caGCAAAAATTGGTAACCC‑GG‑AATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAac > 2:15697/1‑90 (MQ=255) gTAACCC‑GG‑AATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGc > 2:260381/1‑90 (MQ=255) cccc‑GA‑AATCCTTCACTAATATCAATTTTCGAAACCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAg < 1:509116/89‑1 (MQ=255) ccc‑GGAAATACTCCAATAATATAAAATTTCGAAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAg < 1:380331/90‑1 (MQ=255) ttCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATc > 2:186567/1‑90 (MQ=255) tccACTAATATCGATTTTCGAAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATc < 1:549064/88‑1 (MQ=255) ttaatatAGATTTTCGAACCCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCgcgg < 2:233923/87‑1 (MQ=255) atGATATCAATTTTCGAACCCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCgcgg < 1:609639/89‑1 (MQ=255) tatagCGATTTTCGAAACCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCgcggcg < 2:258680/89‑1 (MQ=255) atatCAATTTTCGAAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGa < 1:108179/90‑1 (MQ=255) ctCGAATTAGGAACGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGAcc < 1:427990/89‑1 (MQ=255) atCGAATTTCGAAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGAcc < 1:65046/90‑1 (MQ=255) atCGAATTTCGAAACCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGAcc < 1:193187/90‑1 (MQ=255) aTTTCGAAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCatat < 2:182183/90‑1 (MQ=255) | CAGGATCTGAAAATTGCCGTTATTGGGCTGACAACCGATGACACAGCAAAAATTGGTAACCC‑GG‑AATACTTCACTGATATCGAATTTCGTAAGCCCGCCGATGAAGCGAAGCTGGTGATTCAGGAGCTGCAACAGACAGAAAAGCCAGACATTATTATCGCGGCGACCCATAT > NZ_CP009273/500850‑501022 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |