| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 949,854 | 0 | A | C | 55.6% | 5.8 / 11.7 | 18 | G23G (GGT→GGG) | focA | formate transporter FocA |
| Reads supporting (aligned to +/- strand): ref base A (7/1); new base C (0/10); total (7/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.51e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.08e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAAGACGAATGCGATTGAGATGAAAACACCGGCGGTAATCGCCAGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAA > NZ_CP009273/949770‑949942 | gaagaCGAATGCGATTGAGATGAAAACACCGGCGGTAATCGCCAGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGc > 1:104028/1‑90 (MQ=255) agaCGAATGCGATTGAGATGAAAACACCGGCGGTAATCGCCAGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCct > 1:432047/1‑90 (MQ=255) aCGAATGCGATTGAGATGAAAACACCGGCGGTAATCGCCAGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCctct > 1:47482/1‑90 (MQ=255) aaTGCGATTGAGATGAAAACACCGGCGGTAATCGCCAGATAAAAAAGCTTAAGCGGGGGGTTCGTTGCCTTTTAGACACCCGCCTCTTcc > 2:461185/1‑89 (MQ=255) tGCGATTGAGATAAAACCCCGGGGGGTAACCGCCAGATAAAAAGTCTTAAGGGGTTTTTTCGTTGTTTTATAGACCCCCGCCTCTTCGGc < 2:229564/90‑1 (MQ=255) tGCGATTGAGATAAAAACCCGGGGGGTAACCGCCAGATAGAAAGCCTTAGGGGGTTTTTTCGTTGTTTTAAAGCCCCCCGCCTCTTCGGc < 2:321636/90‑1 (MQ=255) ggCGATGGAGATAAAACCCCCGGGGTAAACCCCCAGAAAAAAAGTTTTAGGGGGTTTTTTCTTTTCTTTAAAGACCCCCGCCTCTTCGGc < 2:399890/89‑1 (MQ=255) gCGATTGAGATGAAAACACCGGCGGTAATCGCCAGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGcc > 2:36076/1‑90 (MQ=255) aaaaaCCCCGGCGGTAACCGCCAGAAAAAAATTCTAAAGGGGATTTTTCGTTGTTTTATAGACCCCCGCCTCTTCGGCCACTTTGGCCAt < 1:325766/89‑1 (MQ=255) ccGGCGGTAATCGCCAGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGGCACCCGCCTCTTCGGCCACTTTGGCCATTgcagca > 2:33837/1‑90 (MQ=255) gcggTAATCGCCAGATAGAAAGTTTTAAGCGGATTTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGa < 1:36076/90‑1 (MQ=255) caccccAGAAAAAAGGTTTTAAGGGGATTTTTGGTTGTTTTAAAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTaaa < 2:89806/86‑1 (MQ=255) cGCCAGATAGAAAGTCTTAAGCGGTTTTTTCGTTGCTTTATAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAg < 1:189502/90‑1 (MQ=255) aTAGAAAGTCTTAAGCGGTTTTTTCGTTGTTTTATAGACCCCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCaaa < 1:96785/90‑1 (MQ=255) gAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAgg > 1:132170/1‑90 (MQ=255) ggttGTTTCTTTGTTTAAAAAACCCCCGCCTCTTCGTCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTc < 2:55420/87‑1 (MQ=255) ggATGTTTCTTTGCTTTATAGACCCCCGCCTTTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTc < 2:62259/90‑1 (MQ=255) ttGTTTTAAAGCCCCCCGCCTCTTTGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAActc < 2:104028/90‑1 (MQ=255) ccacCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTttatta < 1:392002/89‑1 (MQ=255) cacCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGa > 1:154700/1‑38 (MQ=255) cacCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTaa > 2:167460/1‑90 (MQ=255) cacCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTaa > 1:409276/1‑90 (MQ=255) cacCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTaa > 1:349575/1‑90 (MQ=255) cacCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTaa > 1:137124/1‑90 (MQ=255) | GAAGACGAATGCGATTGAGATGAAAACACCGGCGGTAATCGCCAGATAGAAAGTCTTAAGCGGATGTTTCGTTGCTTTATAGACACCCGCCTCTTCGGCCACTTTGGCCATTGCAGCAGGAAGTAAAAGATCAAAAGGGTTGTCAGCTTTCACACTAACTCTCTCTTTATTAA > NZ_CP009273/949770‑949942 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |