| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,024,783 | 0 | T | G | 61.5% | 5.8 / 10.4 | 13 | T215P (ACC→CCC) | rlmI | 23S rRNA (cytosine(1962)‑C(5))‑methyltransferase RlmI |
| Reads supporting (aligned to +/- strand): ref base T (1/4); new base G (8/0); total (9/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCAACCGCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCA > NZ_CP009273/1024697‑1024857 | gCAACCGCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGtggg > 2:525170/1‑87 (MQ=255) aaCCGCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGGAGGCa > 2:67588/1‑90 (MQ=255) gCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGGAGCCAgggg > 1:167344/1‑88 (MQ=255) gCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGGAGCCAgggg > 1:194919/1‑88 (MQ=255) aTCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGGAGCCAcggggcga > 1:453754/1‑88 (MQ=255) ccGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCt < 2:451404/90‑1 (MQ=255) accaccGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGgtagta < 1:74676/90‑1 (MQ=255) gagaAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGGAGCCAGGCGGCTATCACGCTGGGCCAGGGAGTAGCCCGCTTTttgt > 1:83814/1‑90 (MQ=255) aaCAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGGAGGCAGGGGGCCATCACGCTGGTCCCGGGAGGAGGCCGGTTTTTGTTCGt > 2:116141/1‑90 (MQ=255) aaCAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGGAGCCCGGCGGCGATCACCCCGGTCCCGGGAGTAGCCCCGTTTttgttcct > 1:227893/1‑86 (MQ=255) cAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGc < 1:116141/90‑1 (MQ=255) gCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAatat < 2:487313/90‑1 (MQ=255) cGTTTATTTTCAACGTAGCGGCGGGGAGCCACGCGGGGATCCCGCCGGTCCCGGGAGGAACCCGGTTTTTGTCCGTGCTGGATATccacc > 1:187815/1‑90 (MQ=255) cAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCa > 1:161569/1‑90 (MQ=255) | GCAACCGCCCATCAGTGCCGATACGGCGAAACCACCGGTATAGGAGAAACAGTTCAGCACACGTTTATTTTCAACGTAGCGGCGGGTAGCCAGGCGGCTATCACGCTGGTCCAGGTAGTAGCCCGTTTTGTGTCCGTGCTGAATATCCACCAGCAGTTTCA > NZ_CP009273/1024697‑1024857 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |