Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,615,3750AC54.5% 3.0 / 11.5 11V7G (GTG→GGG) eamAO‑acetylserine/cysteine exporter
Reads supporting (aligned to +/- strand):  ref base A (5/0);  new base C (0/6);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

ATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAAC  >  NZ_CP009273/1615327‑1615458
                                                |                                                                                   
atgaCCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATc                                            >  1:217405/1‑90 (MQ=255)
atgaCAACAAAATTTACCCCCCATACGACCCCTACCAGTAGCGCCAACCCCCCACCTTTTCGCGACATTCTACCGCCTCTGAATTTCATc                                            <  1:495787/90‑1 (MQ=255)
    ccACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCtttt                                        >  2:79147/1‑90 (MQ=255)
            tttAGCCCCCATACGCCCACTCCCAGTAGCGCCAACCCCCCATTTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCaa                                <  2:39274/90‑1 (MQ=255)
                          gACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGaa                  >  2:546864/1‑90 (MQ=255)
                              aCTACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTa                                     <  1:528805/67‑1 (MQ=255)
                              aCTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTa                                     >  2:528805/1‑67 (MQ=255)
                               cTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTAc             >  2:211851/1‑90 (MQ=255)
                                tACCAGTAGCGCCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACt            <  2:132697/90‑1 (MQ=255)
                                        gcgcCAACCCCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTa    <  1:529967/90‑1 (MQ=255)
                                          cccAACCCCCCATTTTTTCGCGACTTTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAAc  <  2:396191/89‑1 (MQ=255)
                                                |                                                                                   
ATGACCACAAAATTTAGCCCCCATACGACCACTACCAGTAGCGCCAACACCCCATCTTTTCGCGACATTCTACCGCCTCTGAATTTCATCTTTTGTAAGCAATCAACTTAGCTGAATTTACTTTTCTTTAAC  >  NZ_CP009273/1615327‑1615458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑