| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,367,400 | 0 | A | C | 56.5% | 17.0 / 24.5 | 23 | G361G (GGT→GGG) | menE | o‑succinylbenzoate‑‑CoA ligase |
| Reads supporting (aligned to +/- strand): ref base A (7/3); new base C (0/13); total (7/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.89e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.06e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAG > NZ_CP009273/2367314‑2367485 | aaCTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATAccc < 1:152499/90‑1 (MQ=255)aaCTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATAccc < 2:602653/90‑1 (MQ=255) ttGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGGGAAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCg > 2:328353/1‑90 (MQ=255) gTCGGCAACGGGGACGATAAACCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGcc < 2:574934/90‑1 (MQ=255) cGGCAACGGGGACGATAAACCCCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCAc < 2:214487/90‑1 (MQ=255) ggCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACt < 1:418767/90‑1 (MQ=255) gCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTg < 2:153780/90‑1 (MQ=255) cGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGAt < 1:191293/90‑1 (MQ=255) cTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACg > 2:240779/1‑90 (MQ=255) cAAACCCGCAGGATGTGCAGCAATTACGCGCTCGCCTTCTTCCGGCTGAATCCCCCCTCCGCCACTGAAGAATAGATTGTCTAAACGTcc < 2:567096/90‑1 (MQ=255) cAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTcc < 2:106726/90‑1 (MQ=255) cGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAAt > 1:324393/1‑90 (MQ=255) gCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATg > 1:286991/1‑90 (MQ=255) ggATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTc < 2:244189/90‑1 (MQ=255) tgCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCtt < 1:247995/90‑1 (MQ=255) aagcaATTGCGCGGTCGGTTTCTTCCGGGTGAATCCCCTCGCCCCCAATGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGc < 1:374385/89‑1 (MQ=255) agcaATTACGCGCTCGCCTTCTTCCGGCTGAATCCCTTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGcc < 1:55377/90‑1 (MQ=255) tACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATg > 1:651580/1‑90 (MQ=255) cgcTCGACTTCTTCCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCAtc < 2:579650/90‑1 (MQ=255) ttcCGGCTGAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATc < 1:62134/90‑1 (MQ=255) gCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATcgcgcg > 1:490726/1‑90 (MQ=255) gAATCCCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCgcgtag < 1:4223/90‑1 (MQ=255) gAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCgcgtag > 1:598948/1‑90 (MQ=255) | AACTCCTTGTCGGCAACGGGGACGATAAACACCTGCAAAACCGCAGGATGTGCAGCAATTACGCGCTCGACTTCTTCCGGCTGAATACCCTCTCCGCCACTGAAGAATAGATTGTCTAAACGTCCGACAATGGTCAGCTTGCCATTATGCATCTCACCGCGATCGCGCGTAG > NZ_CP009273/2367314‑2367485 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |