Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092733,659,0580TC55.6% 16.5 / 25.4 27D38G (GAT→GGT) gadXacid resistance transcriptional activator GadX
Reads supporting (aligned to +/- strand):  ref base T (8/4);  new base C (0/15);  total (8/19)
Fisher's exact test for biased strand distribution p-value = 2.23e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-04
Rejected as consensus: Frequency below/above cutoff threshold.

GAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAC  >  NZ_CP009273/3658973‑3659138
                                                                                     |                                                                                
gAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGc                                                                              >  1:460588/1‑90 (MQ=255)
     tCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCaaaaa                                                                         >  2:552627/1‑90 (MQ=255)
     tCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCaaaaa                                                                         >  1:543612/1‑90 (MQ=255)
            aaaTAATAAAGGCGTGTCCCTTGACACAAATAAAAAATTTTCAACCCCCTTATCTCCTCGATTTTGGCTTGCATCCGAAAAAACCAGGTc                                                                  <  2:84048/90‑1 (MQ=255)
                aatAAAAGCGTGTCCCTTGCCCCAAAAAAAAAATTTTCAACCCACTTATCTACTCAATTTTGGCTTGCACCCACAAAACCCAGGTCACCg                                                              <  2:403146/90‑1 (MQ=255)
                     aaGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCAtt                                                         >  2:621052/1‑90 (MQ=255)
                         gtgtCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCa                                                                      <  2:540372/73‑1 (MQ=255)
                         gtgtCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCa                                                                      >  1:540372/1‑73 (MQ=255)
                           gtCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATa                                                   >  2:5474/1‑90 (MQ=255)
                             cctttGACACAAATAAAAAATTTTCAACACACTTATCTCCTCGATTTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGc                                                 <  1:5682/87‑1 (MQ=255)
                             cccTTGACACGAATACAAAATTTTCAACACCTTTATCTCCTCGAATTTGGCTTCCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGc                                                 <  1:215922/90‑1 (MQ=255)
                               cTTGACACAAATACAAAATTTTCAACACCCTTATCTACTCGAATTTGGCTTGCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGa                                               <  1:191632/90‑1 (MQ=255)
                                    cacaAATAAAAATTTTCCACCACATTTTTCTCCTCGATTTTGGCTTGCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTc                                          <  1:44273/90‑1 (MQ=255)
                                        aaTACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCa                                      >  2:291367/1‑90 (MQ=255)
                                          aaaaaatTTTTCAACCCATTTTTCTCCTCGATTTTGGTTTGCACCCAAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCAtt                                    <  1:551941/87‑1 (MQ=255)
                                                  tttCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATgg                            >  2:544604/1‑90 (MQ=255)
                                                   ttCAACACTCTTATCTACTCGATTTTGGCTTCCACCCAAAAAAACCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGt                           <  1:440602/90‑1 (MQ=255)
                                                    tCAACACCTTTATCTACTCGAATTTGGCTTGCACCCAAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                          <  1:291367/90‑1 (MQ=255)
                                                    tCAACACCCTTATCTACTCGATTTTGGCTTGCATCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                          <  1:5474/90‑1 (MQ=255)
                                                    ccAACACATTTATCTACTAAATTTTGGTTTCCACCCAAAAAAACCAGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                          <  2:499788/89‑1 (MQ=255)
                                                         ccacTTTTCTCCTCAATTTTGGCTTCCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat                     <  1:196903/89‑1 (MQ=255)
                                                          cacTTATCTACTCAATTTTGGTTTCCACCCAAAAAACCCGGGCCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAata                    <  2:207792/90‑1 (MQ=255)
                                                                tCTACTGGATTTGGGCTTGCACCCGAAAAAACCAGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTa              <  1:621052/90‑1 (MQ=255)
                                                                            tGGTTTCCACCCGAAAAACCCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAc  <  1:365226/90‑1 (MQ=255)
                                                                            tGGCTTCCACCCGAAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAc  <  1:172918/90‑1 (MQ=255)
                                                                            tGGCTTCCACCCAAAAAACCCAGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAc  <  1:413536/90‑1 (MQ=255)
                                                                            tGGCTCGCACCCAAAAAACCCAGGTCCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAc  <  1:518228/90‑1 (MQ=255)
                                                                                     |                                                                                
GAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAATACAAAATTTTCAACACACTTATCTACTCGAATTTGGCTTGCATCCGCAAAAACCAGGTCACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATAC  >  NZ_CP009273/3658973‑3659138

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: