| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,806,432 | 0 | A | C | 56.2% | 13.8 / 19.2 | 16 | T114T (ACA→ACC) | coaBC | bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate‑‑cysteine ligase CoaBC |
| Reads supporting (aligned to +/- strand): ref base A (6/1); new base C (0/9); total (6/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCT > NZ_CP009273/3806347‑3806516 | ttCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTg < 1:51033/90‑1 (MQ=255)ttCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTg > 1:70504/1‑90 (MQ=255) gCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAAc > 1:633688/1‑90 (MQ=255) gCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAAc > 2:80310/1‑90 (MQ=255) ccGTTTTGCTGCCGGAATGGCGAATGCCCTGGTATCGACGTTTTGTCTGGCTACCCCTGCGCCTGTAGCCGTGCCCCCCGCCATGAACca < 2:633688/90‑1 (MQ=255) gtgtTGCGGCCGGAATGGAGAATCCCGTGGTCCCGCCGATTTGTCTGGCTACCCCTGCGCCTGTAGCCGGCCCCCCCGCCATGAACcagc < 2:419041/90‑1 (MQ=255) ttGCTGCCGGAATGGCAAATGCCCTGGTATCGACGTTTTGTCTGGCTACCCCTGCGCCTGTAGCCGTGCCCCCCGCCATGAACCAGCAGa < 2:93964/90‑1 (MQ=255) ttGCTCCCGGAATGGCGAATGCCCTGGTATCGACGATTTGTCTGGCTCCCCCTGCCCCTGTAGCCGTGCCCCCCGCCATGAACCAGCAGa < 1:535001/90‑1 (MQ=255) tgcCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTa > 2:550608/1‑90 (MQ=255) tgcccTGGTACCGACGATTTGCCTGGCTACCCCTGCGCCTGTAGCCGTGCCCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCAc < 2:330498/87‑1 (MQ=255) cccTGGTATCGACGATTTGTCTGGCTACCCCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACgc < 1:561690/89‑1 (MQ=255) gacgaTTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAAttt > 1:382426/1‑90 (MQ=255) gacgaTTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAAttt > 2:62524/1‑90 (MQ=255) gttttGTCGGGCTACCCCTGCGCCTGTGGCCGTGCCCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTaga < 2:376090/88‑1 (MQ=255) tttGTCGGGCTACCCCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAgg < 1:122754/90‑1 (MQ=255) gCTACCCCTGCCCCTGTAGCCGTGCCCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGtgcttgct < 1:615515/90‑1 (MQ=255) | TTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGTGCTTGCT > NZ_CP009273/3806347‑3806516 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |