Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,941,604	A→C	G10G (GGT→GGG)	ruvC ←	crossover junction endodeoxyribonuclease RuvC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,941,604	0	A	C	100.0%	81.4 / NA	26	G10G (GGT→GGG)	ruvC	crossover junction endodeoxyribonuclease RuvC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (14/12);  total (14/12)

TTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGAACCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGACCTCA  >  NZ_CP009273/1941524‑1941689                                                                                 |                                                                                      ttGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCa                                                                              >  2:322272/1‑90 (MQ=255)  tGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCaa                                                                             <  1:597559/90‑1 (MQ=255)       cGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCg                                                                        <  2:363522/90‑1 (MQ=255)                 cGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAg                                                              <  1:132360/90‑1 (MQ=255)                   cTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGGATAATAGcc                                                            >  1:166508/1‑90 (MQ=255)                       ccAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCa                                                        <  2:557569/90‑1 (MQ=255)                          ggTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCAcgc                                                     >  2:146362/1‑90 (MQ=255)                             aGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTGGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGtc                                                  <  2:216179/90‑1 (MQ=255)                                aCAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCg                                                                        <  2:14421/65‑1 (MQ=255)                                aCAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCg                                                                        >  1:14421/1‑65 (MQ=255)                                   gTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGtt                                            >  2:87225/1‑90 (MQ=255)                                   gTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGt                                             <  1:220924/89‑1 (MQ=255)                                   gTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGt                                             >  2:220924/1‑89 (MQ=255)                                     tGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGtttt                                          >  2:523529/1‑90 (MQ=255)                                       cctacctaCCTGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGc                                        <  1:9427/90‑1 (MQ=255)                                        ctacctacCTGGCGGATCACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGGCTCCGGTTTGCt                                       >  2:69283/1‑90 (MQ=255)                                             tacctGGCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGGTTTGCTGTTTa                                  >  1:465335/1‑90 (MQ=255)                                                   gCGGATGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGc                            <  2:243049/90‑1 (MQ=255)                                                        tGACGCCGTAGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCtcat                       >  1:211881/1‑90 (MQ=255)                                                                 aGCCGGTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTc              <  2:527258/90‑1 (MQ=255)                                                                     ggTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGt               <  2:228180/85‑1 (MQ=255)                                                                     ggTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGt               >  1:228180/1‑85 (MQ=255)                                                                     ggTCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTg          >  1:124597/1‑90 (MQ=255)                                                                       tCACGCGCGACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCg        >  1:276161/1‑90 (MQ=255)                                                                          cgcgcgACCCAGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGAcc     <  2:57943/90‑1 (MQ=255)                                                                             gcgACCCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGACCtca  >  1:178172/1‑90 (MQ=255)                                                                                 |                                                                                      TTGGTGCGGATGCATCCGCTACCCAGGTAGGACAGTTGCCTACCTACCTGGCGGATGACGCCGTAGCCGGTCACGCGCGAACCCGGATCAATGCCGAGAATAATAGCCATCACGCGTCTCCGTTTTGCTGTTTAGCAGGCCTCATCAGAGAGTCGCTGCGACCTCA  >  NZ_CP009273/1941524‑1941689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑