Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	231,042	A→C	G76G (GGT→GGG)	gloB ←	hydroxyacylglutathione hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	231,042	0	A	C	100.0%	69.4 / NA	22	G76G (GGT→GGG)	gloB	hydroxyacylglutathione hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (7/15);  total (7/15)

TTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGACCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATGGTGGGTGA  >  NZ_CP009273/230957‑231118                                                                                      |                                                                             ttACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCAt                                                                          >  1:265009/1‑90 (MQ=255)   acacTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATac                                                                        <  1:159676/90‑1 (MQ=255)        aaaTTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATAcaccac                                                                   >  1:583624/1‑90 (MQ=255)        aaaTTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATAcaccac                                                                   >  1:455458/1‑90 (MQ=255)                   ccAAAACGAAGGCTGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGaa                                                        <  2:573229/90‑1 (MQ=255)                    cAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGaaa                                                       <  1:479951/90‑1 (MQ=255)                              gCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTccacc                                             <  2:331790/90‑1 (MQ=255)                                           cTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTAcgc                                <  1:562500/90‑1 (MQ=255)                                               gACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACgccgcc                            <  1:157773/90‑1 (MQ=255)                                                   aCCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACg                        >  2:606415/1‑90 (MQ=255)                                                         gttgttCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCg                  <  1:322285/90‑1 (MQ=255)                                                          ttgttCCCTTATCTTGTGTCTCTTGTGGCCCATACACCCCAATTTGTGGCAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCgt                 <  1:31678/90‑1 (MQ=255)                                                          ttgttCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCgt                 <  1:186439/90‑1 (MQ=255)                                                              tCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCgtggtg             >  1:420639/1‑90 (MQ=255)                                                               cccTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGa            <  1:465345/90‑1 (MQ=255)                                                                   tATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGAtggt        <  2:455458/90‑1 (MQ=255)                                                                      cTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATggtggg     <  2:104010/90‑1 (MQ=255)                                                                        tgtgTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATggtgggtg   <  1:503273/90‑1 (MQ=255)                                                                        tgtgTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATggtgggtg   <  2:272850/90‑1 (MQ=255)                                                                        tgtgTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATggtgggtg   <  1:280084/90‑1 (MQ=255)                                                                         gtgtCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGCTCGTGGTGATGGTGGGTga  >  2:560714/1‑90 (MQ=255)                                                                         gtgtCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATGGTGGGTga  >  2:161722/1‑90 (MQ=255)                                                                                      |                                                                             TTACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGACCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATGGTGGGTGA  >  NZ_CP009273/230957‑231118

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑