| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,003,480 | T→C | F60S (TTC→TCC) | yedK → | SOS response‑associated peptidase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,003,480 | 0 | T | C | 96.0% | 72.9 / NA | 25 | F60S (TTC→TCC) | yedK | SOS response‑associated peptidase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (8/16); minor base A (1/0); total (9/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.60e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AACCCATTGGCAGATACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTTCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTATGTTTAAAC > NZ_CP009273/2003393‑2003566 | aaCCCATTGGCAGATACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCt < 2:133799/90‑1 (MQ=255) cATTGGCAGATACAACGTCGCGCCCGCGACTAAAGTCCTACTGCTCAATACACGTGATGAACACCTTCCTCTGACTCCGGTTTACTgctg > 2:335011/1‑87 (MQ=255) ttGGCAGATACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGat > 1:468760/1‑90 (MQ=255) tGGCAGATACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGata < 2:630281/90‑1 (MQ=255) gCAGATACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGATATg < 2:165714/90‑1 (MQ=255) tACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGATATGCTccc < 2:115519/90‑1 (MQ=255) gTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGATATGCTCCCGGAtgg < 2:272461/90‑1 (MQ=255) gcgcCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGatat > 2:75781/1‑75 (MQ=255) gcgcCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGatat < 1:75781/75‑1 (MQ=255) gcgcCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGATATGCTCCCGGAtggtgg > 1:250909/1‑90 (MQ=255) aaGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGATATGCTCCCGGATGGTGGGATAAACcgccgc < 2:140275/90‑1 (MQ=255) tGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGATATGCTCCCGGATGGTGGGACAAACCGCCGCTGATTAACg > 2:61440/1‑90 (MQ=255) gCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGc > 1:568653/1‑90 (MQ=255) tCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGatat < 1:543693/50‑1 (MQ=255) tCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGatat > 2:543693/1‑50 (MQ=255) tCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTCCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGccc < 1:82083/90‑1 (MQ=255) acCTTCATCTGGATCCGGTTTCCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCaa > 2:365258/1‑90 (MQ=255) cTTCATCTGGATCCGGTTTCCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAGACTGCGGCAAcc < 1:278566/90‑1 (MQ=255) cTTCATCTGGATCCGGTTTCCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAAcc < 1:558745/90‑1 (MQ=255) cTTCATCTGGATCCGGTTTCCTGGGGATATGCCCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAAcc < 2:477458/90‑1 (MQ=255) cTGGATCCGGTTTCCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGt < 1:515513/90‑1 (MQ=255) tGGATCCGGTTTCCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTa < 1:577745/90‑1 (MQ=255) tGGATCCGGTTTCCTGGGGATATCCCCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTa < 2:609560/90‑1 (MQ=255) gATCCGGTTTCCTGGGGATATGCTCCCGGATGGTGGGAt < 2:45945/39‑1 (MQ=255) gATCCGGTTTCCTGGGGATATGCTCCCGGATGGTGGGAt > 1:45945/1‑39 (MQ=39) tttcCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTATGTTTAAAc < 2:568653/90‑1 (MQ=255) | AACCCATTGGCAGATACAACGTCGCGCCGGGAACCAAAGTCCTACTGCTCAGTGAACGTGATGAACACCTTCATCTGGATCCGGTTTTCTGGGGATATGCTCCCGGATGGTGGGATAAACCGCCGCTGATTAACGCCCGCGTAGAAACTGCGGCAACCAGTCGTATGTTTAAAC > NZ_CP009273/2003393‑2003566 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |