Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,174,381	T→G	C270G (TGC→GGC)	yegU →	ADP‑ribosylglycohydrolase family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,174,381	0	T	G	100.0%	88.4 / NA	28	C270G (TGC→GGC)	yegU	ADP‑ribosylglycohydrolase family protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (15/13);  total (15/13)

GTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGC  >  NZ_CP009273/2174297‑2174464                                                                                     |                                                                                    gTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGcc                                                                                >  1:186383/1‑90 (MQ=255)    gTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTc                                                                             >  1:19195/1‑90 (MQ=255)     ttGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTcc                                                                            >  1:559474/1‑90 (MQ=255)       ggCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCtg                                                                          <  1:68211/90‑1 (MQ=255)                       cTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTggcg                                                          >  1:203505/1‑90 (MQ=255)                       cTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTggcg                                                          >  1:90933/1‑90 (MQ=255)                         aTTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTggcggc                                                        >  2:200152/1‑90 (MQ=255)                              gTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGacac                                                   >  2:298538/1‑90 (MQ=255)                              gTCCGTTCCGTGCGCCATTGCGCTGGTTGAACCGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGacac                                                   >  1:656204/1‑90 (MQ=255)                                 cgttccgtGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGa                                                >  2:469239/1‑90 (MQ=255)                                   ttccgtGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGaca                                              >  2:472371/1‑90 (MQ=255)                                   ttccgtGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGaca                                              <  2:254578/90‑1 (MQ=255)                                        tGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATc                                         <  2:186383/90‑1 (MQ=255)                                        tGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATc                                         <  1:469239/90‑1 (MQ=255)                                        tGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATc                                         <  1:213117/90‑1 (MQ=255)                                        tGCGCCATCGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATc                                         <  2:205502/90‑1 (MQ=255)                                                    cTGGTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCg                             <  2:19195/90‑1 (MQ=255)                                                      ggTTGAACTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGAc                           >  1:113184/1‑90 (MQ=255)                                                            aCTGGCACAAACCGACCCGAATCGCGGCGCCGTCCTTTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAAt                     >  2:301475/1‑90 (MQ=255)                                                                         gACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTg                                                             >  2:358658/1‑37 (MQ=255)                                                                         gACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTg                                                             <  1:358658/37‑1 (MQ=255)                                                                         gACCCGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTg        >  2:529991/1‑90 (MQ=255)                                                                            ccGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCAt     <  1:533908/90‑1 (MQ=255)                                                                             ccaaTCGCGGCGCCGTCCGGTGCGCTAACCTTGGCGGCGACACCGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATg    <  2:575409/88‑1 (MQ=255)                                                                             cGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATg    <  1:627228/90‑1 (MQ=255)                                                                             cGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATg    <  2:250450/90‑1 (MQ=255)                                                                             cGAATCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATg    <  1:523940/90‑1 (MQ=255)                                                                               aaTCGCGGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGc  >  2:427730/1‑90 (MQ=255)                                                                                     |                                                                                    GTCGTTGGCGCAGGTACCAGCACTATTGAGTCCGTTCCGTGCGCCATTGCGCTGGTTGAACTGGCACAAACCGACCCGAATCGCTGCGCCGTCCTGTGCGCTAACCTTGGCGGCGACACAGACACCATCGGTGCTATGGCGACGGCAATTTGCGGCGCGTTGCATGGC  >  NZ_CP009273/2174297‑2174464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑