Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,536,106	T→C	E261G (GAA→GGA)	cysP ←	thiosulfate/sulfate ABC transporter substrate‑binding protein CysP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,536,106	0	T	C	100.0%	92.8 / NA	29	E261G (GAA→GGA)	cysP	thiosulfate/sulfate ABC transporter substrate‑binding protein CysP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/6);  total (23/6)

TATTCACGCGGTAGTAATAGTCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTTCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTCGGAATCACCACTTCAA  >  NZ_CP009273/2536023‑2536190                                                                                    |                                                                                     tATTCACGCGGTAGTAATAGTCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTAc                                                                                >  2:656064/1‑90 (MQ=255) tATTCACGCGGTAGTAATAGTCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTAc                                                                                >  2:336716/1‑90 (MQ=255)  aTTCACGCGGTAGTAATAGTCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTAcc                                                                               >  2:400321/1‑90 (MQ=255)  aTTCACGCGGTAGTAATAGTCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTAcc                                                                               >  2:209365/1‑90 (MQ=255)       cgcgGTAGTAATAGTCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTgg                                                                          >  1:498946/1‑90 (MQ=255)                   aGTCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGtttt                                                              >  2:109075/1‑90 (MQ=255)                    gTCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGttttt                                                             >  1:356178/1‑90 (MQ=255)                     tCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTa                                                            >  1:268039/1‑90 (MQ=255)                     tCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTa                                                            >  2:479309/1‑90 (MQ=255)                     tCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTa                                                            >  2:336722/1‑90 (MQ=255)                     tCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTa                                                            >  2:284508/1‑90 (MQ=255)                     tCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTa                                                            >  2:130162/1‑90 (MQ=255)                       ggTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATc                                                          >  1:283424/1‑90 (MQ=255)                                ggTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGc                                                 >  1:449335/1‑90 (MQ=255)                                     gcgcCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCg                                            >  2:376953/1‑90 (MQ=255)                                           gCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGc                                                 >  1:515757/1‑79 (MQ=255)                                           gCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGc                                                 <  2:515757/79‑1 (MQ=255)                                           gCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAAtt                                      >  2:540439/1‑90 (MQ=255)                                                  ataGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAg                               <  1:376953/90‑1 (MQ=255)                                                  ataGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAg                               <  2:356178/90‑1 (MQ=255)                                                         cAGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTg                        >  2:484905/1‑90 (MQ=255)                                                          aGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTgg                       >  1:595822/1‑90 (MQ=255)                                                          aGTTCAGATAGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTgg                       >  1:162199/1‑90 (MQ=255)                                                                   aGGCTTTGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTCGGaa              <  2:76572/90‑1 (MQ=255)                                                                        ttGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTCGGAATcacc         <  1:336716/90‑1 (MQ=255)                                                                        ttGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTCGGAATcacc         <  1:656064/90‑1 (MQ=255)                                                                        ttGGCGGCTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTCGGAATcacc         >  2:145841/1‑90 (MQ=255)                                                                              gcTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTCGGAATCACCACTTCa   >  2:476457/1‑90 (MQ=255)                                                                               cTTTTCCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCGGCCAGAATGTTGGTTTTCGGAATCACCACTTCaa  >  2:475211/1‑90 (MQ=255)                                                                                    |                                                                                     TATTCACGCGGTAGTAATAGTCGGTGATGATGGTTTGCGCCTGCGGGCTATAGAGCCAGTTCAGATAGGCTTTGGCGGCTTTTTCCGTACCGTTGGCCTGCACGTTTTTATCAACCCACGCCACCGGGAATTCCGCCAGAATGTTGGTTTTCGGAATCACCACTTCAA  >  NZ_CP009273/2536023‑2536190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑