Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	297,509	G→C	G30G (GGC→GGG)	paoB ←	aldehyde oxidoreductase FAD‑binding subunit PaoB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	297,509	0	G	C	100.0%	57.1 / NA	19	G30G (GGC→GGG)	paoB	aldehyde oxidoreductase FAD‑binding subunit PaoB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (10/9);  total (10/9)

ATCGAGCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAA  >  NZ_CP009273/297428‑297588                                                                                  |                                                                                aTCGAGCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGAt                                                                         >  2:542609/1‑90 (MQ=255) aTCGAGCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGAt                                                                         >  2:158793/1‑90 (MQ=255)             gCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGcc                                                             <  1:264773/90‑1 (MQ=255)             gCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGcc                                                             <  2:447287/90‑1 (MQ=255)                gTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCggg                                                          >  1:629682/1‑90 (MQ=255)                gTTCACATCGATAAGGGGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCggg                                                          >  2:636635/1‑90 (MQ=255)                   cacaTCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTAc                                                       <  2:629682/90‑1 (MQ=255)                                    tgggCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCt                                                  >  2:25200/1‑78 (MQ=255)                                    tgggCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGGACGCGCt                                                  <  1:25200/78‑1 (MQ=255)                                       gCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTgccg                                   >  2:290302/1‑90 (MQ=255)                                         gTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTgccgcc                                 >  1:211352/1‑90 (MQ=255)                                              aaTTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGc                            <  1:580724/90‑1 (MQ=255)                                                 ttCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTgg                         <  1:565507/90‑1 (MQ=255)                                                   ccAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTgggg                       <  2:131902/90‑1 (MQ=255)                                                            tcaGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTc              <  1:190717/90‑1 (MQ=255)                                                                  ccAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCAt        <  1:170498/90‑1 (MQ=255)                                                                   cagcagATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATa       >  1:648265/1‑90 (MQ=255)                                                                    agcagATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAg      >  1:649494/1‑90 (MQ=255)                                                                        gATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTaa  >  2:68934/1‑90 (MQ=255)                                                                                  |                                                                                ATCGAGCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAA  >  NZ_CP009273/297428‑297588

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑