Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,785,687	G→C	G75G (GGC→GGG)	kbl ←	glycine C‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,785,687	0	G	C	100.0%	67.0 / NA	22	G75G (GGC→GGG)	kbl	glycine C‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (11/11);  total (11/11)

AGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAGTTGTTGGCACAA  >  NZ_CP009273/3785613‑3785771                                                                           |                                                                                     aGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGaa                                                                       >  1:481924/1‑90 (MQ=255)        ccgccAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTc                                                                >  2:605388/1‑90 (MQ=255)          gccAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTccc                                                              >  1:198283/1‑90 (MQ=255)                       cAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGccgccg                                                 <  1:98128/90‑1 (MQ=255)                       cAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGccgccg                                                 <  1:607593/90‑1 (MQ=255)                           ctctTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAat                                             <  1:486875/90‑1 (MQ=255)                            tctTTATGGCTGTCCTGAGTGCCGCAAAAAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAatc                                            <  2:180854/90‑1 (MQ=255)                              tttATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAatcag                                          >  2:220367/1‑90 (MQ=255)                                 aTGGCTGTCCTGAGTGCCGCAAATAAAACGCCCCGAAGCCACCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAatcagatc                                       <  2:316648/90‑1 (MQ=255)                                 aTGGCTGTCCTGAGTGCCGAAAAAAAAACGCCCCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAatcagatc                                       <  2:481924/90‑1 (MQ=255)                                       gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg                                 >  1:411669/1‑90 (MQ=255)                                       gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg                                 >  1:315881/1‑90 (MQ=255)                                       gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg                                 >  1:654150/1‑90 (MQ=255)                                       gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg                                 >  1:277229/1‑90 (MQ=255)                                           tGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTc                             >  1:472447/1‑90 (MQ=255)                                                cccGCAAATAAAACGCCCCGAAGCCACCCCGAAACCGTGAGAACCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAg                        <  2:418282/89‑1 (MQ=255)                                                 ccGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGc                       >  2:609365/1‑90 (MQ=255)                                                  cGCAAATAAACCGCACCGAAGCCACCCCGAAACCGTGAGAATCCATCCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGcc                      <  2:574225/90‑1 (MQ=255)                                                   gCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGccc                     <  1:220367/90‑1 (MQ=255)                                                   gCAAATAAAACGCACCGAAGCCACCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGccc                     <  1:395333/90‑1 (MQ=255)                                                      aaTAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCgag                  >  2:177102/1‑90 (MQ=255)                                                                      gCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAGTTGTTGGCACaa  <  2:136548/90‑1 (MQ=255)                                                                           |                                                                                     AGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAGTTGTTGGCACAA  >  NZ_CP009273/3785613‑3785771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑