Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,786,467	T→C	I193V (ATT→GTT)	yibB ←	protein YibB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,786,467	0	T	C	100.0%	72.4 / NA	23	I193V (ATT→GTT)	yibB	protein YibB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/4);  total (19/4)

AATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAAT  >  NZ_CP009273/3786394‑3786549                                                                          |                                                                                   aaTTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAAcc                                                                    >  1:257736/1‑90 (MQ=255)                      ttATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTa                                               <  1:140657/90‑1 (MQ=255)                             gtgAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCtt                                        >  2:621397/1‑90 (MQ=255)                                         ttGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGa                            >  1:367649/1‑90 (MQ=255)                                                cccAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATg                     >  1:399467/1‑90 (MQ=255)                                                          tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACtt           >  1:408146/1‑90 (MQ=255)                                                          tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACtt           >  1:521005/1‑90 (MQ=255)                                                          tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACtt           >  2:584806/1‑90 (MQ=255)                                                          tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACtt           >  1:596116/1‑90 (MQ=255)                                                          tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACtt           <  1:108335/90‑1 (MQ=255)                                                          tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACtt           >  2:338245/1‑90 (MQ=255)                                                            taCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtc         >  1:76960/1‑90 (MQ=255)                                                              cATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGcc                                           >  1:636938/1‑54 (MQ=255)                                                              cATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGcc                                           <  2:636938/54‑1 (MQ=255)                                                              cATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcat       >  2:454093/1‑90 (MQ=255)                                                              cATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcat       >  1:570871/1‑90 (MQ=255)                                                              cATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcat       >  1:555685/1‑90 (MQ=255)                                                              cATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcat       >  2:641006/1‑90 (MQ=255)                                                                 gATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCaa    >  2:414519/1‑90 (MQ=255)                                                                  aTTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCaaa   >  2:379496/1‑90 (MQ=255)                                                                   ttGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGaa                           <  2:203076/65‑1 (MQ=255)                                                                   ttGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGaa                           >  1:203076/1‑65 (MQ=255)                                                                   ttGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTCTTATAGTGAAAAGATGCATCTTACTTTCATCAAAt  >  2:403711/1‑90 (MQ=255)                                                                          |                                                                                   AATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAAT  >  NZ_CP009273/3786394‑3786549

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑